Anti-MMP-13 Antibody

Product Name

Anti-MMP-13 Antibody

View all MMP-13 Antibodies

SKU/Catalog Number

A00420-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MMP-13 Antibody catalog # A00420-1. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MMP-13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00420-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide of human SPR.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with eNOS or nNOS.

Reactive Species

A00420-1 is reactive to MMP13 in Human, Mouse

Applications

A00420-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

53.82kDa

Background of MMP-13

Isoform 1:Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.

Kan-Tai Hsia, Development, Jan 2003; 130: 369.
Jim Selfridge, Nucleic Acids Res., Nov 2001; 29: 4541 - 4550.
Cha-Kyung Youn, Cancer Res., Jul 2004; 64: 4849 - 4857.
Teresa A. Motycka, J. Biol. Chem., Apr 2004; 279: 13634 - 13639.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of MMP13 using Anti-MMP-13 Antibody (A00420-1).

ICC staining MMP13 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-MMP-13 Antibody A00420-1

Western blot analysis of MMP13 on MCF-7 cells lysates using anti-MMP13 antibody at 1/1

Gene/Protein Information For MMP13 (Source: Uniprot.org, NCBI)

Alternative Names

CLG 3; CLG3EC 3.4.24; collagenase 3; EC 3.4.24.-; EC 3.4.24.22; EC 3.4.24.24; EC 3.4.24.35; EC 3.4.24.65; EC 3.4.24.7; MANDP1; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP13; MMP-13 MMP13 CLG3, MANDP1, MDST, MMP-13 matrix metallopeptidase 13 collagenase 3|matrix metalloproteinase 13 (collagenase 3)

For more info on MMP13, check out the MMP13 Infographic

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In this infographic, you will see the following information for MMP13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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