Anti-MMP13 Rabbit Monoclonal Antibody

MMP-13 antibody

Boster Bio Anti-MMP13 Rabbit Monoclonal Antibody catalog # M00420. Tested in WB application. This antibody reacts with Human, Mouse. Cited in 4 publication(s).

Product Info Summary

SKU: M00420
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-MMP13 Rabbit Monoclonal Antibody

View all MMP-13 Antibodies

SKU/Catalog Number

M00420

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MMP13 Rabbit Monoclonal Antibody catalog # M00420. Tested in WB application. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MMP13 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00420)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

IDA-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human MMP13

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00420 is reactive to MMP13 in Human, Mouse

Applications

M00420 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

53.82kDa

Background of MMP-13

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MMP13 (Source: Uniprot.org, NCBI)

Gene Name

MMP13

Full Name

Collagenase 3

Weight

53.82kDa

Superfamily

peptidase M10A family

Alternative Names

CLG 3; CLG3EC 3.4.24; collagenase 3; EC 3.4.24.-; EC 3.4.24.22; EC 3.4.24.24; EC 3.4.24.35; EC 3.4.24.65; EC 3.4.24.7; MANDP1; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP13; MMP-13 MMP13 CLG3, MANDP1, MDST, MMP-13 matrix metallopeptidase 13 collagenase 3|matrix metalloproteinase 13 (collagenase 3)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MMP13, check out the MMP13 Infographic

MMP13 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MMP13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00420 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chondroprotective effects of ginsenoside Rg1 in human osteoarthritis chondrocytes and a rat model of anterior cruciate ligament transection

Hydrogen sulfide attenuates IL-1%u03B2-induced inflammatory signaling and dysfunction of osteoarthritic chondrocytes

Effects of ultrasound on estradiol level, bone mineral density, bone biomechanics and matrix metalloproteinase-13 expression in ovariectomized rabbits

Huang Gf, Zou J, Shi J, Zhang Dy, Pen Hf, Zhang Q, Gao Y, Wang By. Evid Based Complement Alternat Med. 2014;2014:731395. Doi: 10.1155/2014/731395. Epub 2014 May 27. The Effect Of Electroacupuncture On The Extracellular Matrix Synthesis And Degrada...

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15 Customer Q&As for Anti-MMP13 Rabbit Monoclonal Antibody

Question

Please see the WB image, lot number and protocol we used for mammary carcinoma using anti-MMP13 Rabbit Monoclonal antibody M00420. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-26

Question

I see that the anti-MMP13 Rabbit Monoclonal antibody M00420 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-26

Question

I would like to test anti-MMP13 Rabbit Monoclonal antibody M00420 on mouse mammary carcinoma for research purposes, then I may be interested in using anti-MMP13 Rabbit Monoclonal antibody M00420 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-25

Answer

The products we sell, including anti-MMP13 Rabbit Monoclonal antibody M00420, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-25

Question

We are currently using anti-MMP13 Rabbit Monoclonal antibody M00420 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

The anti-MMP13 Rabbit Monoclonal antibody (M00420) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-19

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary carcinoma using anti-MMP13 Rabbit Monoclonal antibody M00420. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-10

Question

I was wanting to use your anti-MMP13 Rabbit Monoclonal antibody for WB for mouse mammary carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse mammary carcinoma identification?

M. Jackson

Verified customer

Asked: 2020-02-05

Answer

As indicated on the product datasheet, M00420 anti-MMP13 Rabbit Monoclonal antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse mammary carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-05

Question

Is there a BSA free version of anti-MMP13 Rabbit Monoclonal antibody M00420 available?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP13 Rabbit Monoclonal antibody M00420 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-08

Question

Our lab were happy with the WB result of your anti-MMP13 Rabbit Monoclonal antibody. However we have observed positive staining in lung extracellular space using this antibody. Is that expected? Could you tell me where is MMP13 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

From what I have seen in literature, lung does express MMP13. Generally MMP13 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP13 expression, here are a few articles citing expression in various tissues:
Esophagus, and Synovium, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Mammary carcinoma, Pubmed ID: 8207000, 9562863

Boster Scientific Support

Answered: 2020-01-06

Question

Is a blocking peptide available for product anti-MMP13 Rabbit Monoclonal antibody (M00420)?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

We do provide the blocking peptide for product anti-MMP13 Rabbit Monoclonal antibody (M00420). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-10

Question

Will anti-MMP13 Rabbit Monoclonal antibody M00420 work for WB with mammary carcinoma?

Verified Customer

Verified customer

Asked: 2019-04-10

Answer

According to the expression profile of mammary carcinoma, MMP13 is highly expressed in mammary carcinoma. So, it is likely that anti-MMP13 Rabbit Monoclonal antibody M00420 will work for WB with mammary carcinoma.

Boster Scientific Support

Answered: 2019-04-10

Question

Is this M00420 anti-MMP13 Rabbit Monoclonal antibody reactive to the isotypes of MMP13?

Verified Customer

Verified customer

Asked: 2018-12-05

Answer

The immunogen of M00420 anti-MMP13 Rabbit Monoclonal antibody is A synthesized peptide derived from human MMP13. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-12-05

Question

Will M00420 anti-MMP13 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-02-26

Answer

You can see on the product datasheet, M00420 anti-MMP13 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-26

Question

My question regards using your anti-MMP13 Rabbit Monoclonal antibody for response to amyloid-beta studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-12-05

Answer

Thank you for your inquiry. This M00420 anti-MMP13 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-12-05

Question

We have been able to see staining in human esophagus synovium. What should we do? Is anti-MMP13 Rabbit Monoclonal antibody supposed to stain esophagus synovium positively?

P. Kulkarni

Verified customer

Asked: 2017-08-18

Answer

Based on literature esophagus synovium does express MMP13. Based on Uniprot.org, MMP13 is expressed in tibia, mammary carcinoma, esophagus synovium, lung, among other tissues. Regarding which tissues have MMP13 expression, here are a few articles citing expression in various tissues:
Esophagus, and Synovium, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Mammary carcinoma, Pubmed ID: 8207000, 9562863

Boster Scientific Support

Answered: 2017-08-18

Question

Can you help my question with product M00420, anti-MMP13 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

G. Parker

Verified customer

Asked: 2013-11-21

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00420 anti-MMP13 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-11-21

Order DetailsPrice
M00420

100μL

$370
M00420-30ul

30μL sample

$99
M00420-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M00420
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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