Anti-MRP1/ABCC1 Antibody Picoband™

Product Name

Anti-MRP1/ABCC1 Antibody Picoband™

View all MRP1 Antibodies

SKU/Catalog Number

A00872-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MRP1/ABCC1 Antibody Picoband™ catalog # A00872-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MRP1/ABCC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00872-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MRP1/ABCC1 recombinant protein (Position: S239-K357).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00872-1 is reactive to ABCC1 in Human

Applications

A00872-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

220 kDa

Calculated molecular weight

171.591kDa

Background of MRP1

Multidrug resistance-associated protein 1 (MRP1) is a protein that in humans is encoded by the ABCC1 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MRP1/ABCC1 using anti-MRP1/ABCC1 antibody (A00872-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP1/ABCC1 antigen affinity purified polyclonal antibody (Catalog # A00872-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP1/ABCC1 at approximately 220 kDa. The expected band size for MRP1/ABCC1 is at 220 kDa.

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Figure 2. Flow Cytometry analysis of SiHa cells using anti-MRP1/ABCC1 antibody (A00872-1).
Overlay histogram showing SiHa cells stained with A00872-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MRP1/ABCC1 Antibody (A00872-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ABCC1 (Source: Uniprot.org, NCBI)

Alternative Names

ABC29; ABCC; ABCC1; ATP-binding cassette sub-family C member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; EC 3.6.3; EC 3.6.3.44; GS-X; Leukotriene C(4) transporter; LTC4 transporter; MRP1; MRP1DKFZp781G125; MRPDKFZp686N04233; multidrug resistance associated protein 1; multidrug resistance protein; multidrug resistance-associated protein 1; multiple drug resistance protein 1; multiple drug resistance-associated protein ABCC1 ABC29, ABCC, DFNA77, GS-X, MRP, MRP1 ATP binding cassette subfamily C member 1 multidrug resistance-associated protein 1|ATP-binding cassette transporter variant ABCC1delta-ex13|ATP-binding cassette transporter variant ABCC1delta-ex13&14|ATP-binding cassette transporter variant ABCC1delta-ex25|ATP-binding cassette transporter variant ABCC1delta-ex25&26|ATP-binding cassette, sub-family C (CFTR/MRP), member 1|LTC4 transporter|glutathione-S-conjugate-translocating ATPase ABCC1|leukotriene C(4) transporter

For more info on ABCC1, check out the ABCC1 Infographic

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In this infographic, you will see the following information for ABCC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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