Anti-MUC2 (Mucin 2) Monoclonal Antibody

Product Name

Anti-MUC2 (Mucin 2) Monoclonal Antibody

View all MUC2 Antibodies

SKU/Catalog Number

M01212

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-MUC2 (Mucin 2) Monoclonal Antibody (Catalog # M01212). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-MUC2 (Mucin 2) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01212)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: MLP/842

Isotype

IgG1, kappa

Immunogen

Recombinant fragment covering tandem repeats of human MUC2 protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01212 is reactive to MUC2 in Human

Applications

M01212 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

540.3kDa

Background of MUC2

Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This monoclonal antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium.MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Anti-MUC2 Mouse Monoclonal Antibody (MLP/842).

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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Anti-MUC2 Mouse Monoclonal Antibody (MLP/842).

Gene/Protein Information For MUC2 (Source: Uniprot.org, NCBI)

Alternative Names

Intestinal mucin-2; MLP; MUC-2; mucin 2, intestinal/tracheal; mucin 2, oligomeric mucus/gel-forming; mucin-2; mucin-like protein; SMUC MUC2 MLP, MUC-2, SMUC mucin 2, oligomeric mucus/gel-forming mucin-2|mucin 2, intestinal/tracheal

For more info on MUC2, check out the MUC2 Infographic

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In this infographic, you will see the following information for MUC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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