Click image to see more details
Product Info Summary
| SKU: | M00612-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal Antibody
SKU/Catalog Number
M00612-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal Antibody catalog # M00612-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00612-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABAD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MUC5AC
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00612-1 is reactive to MUC5AC in Human
Applications
M00612-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
32-42 kDa, 65 kDa, 75 kDa
Calculated molecular weight
585.57kDa
Background of MUC5AC
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of MUC5AC expression in human stomach lysate.
Protein Target Info & Infographic
Gene/Protein Information For MUC5AC (Source: Uniprot.org, NCBI)
Gene Name
MUC5AC
Full Name
Mucin-5AC
Weight
585.57kDa
Alternative Names
gastric mucin; leB; lewis B blood group antigen; major airway glycoprotein; MUC5; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin 5AC, oligomeric mucus/gel-forming; mucin-5 subtype AC, tracheobronchial; mucin-5AC; TBM; tracheobronchial mucin MUC5AC MUC5, TBM, leB, mucin mucin 5AC, oligomeric mucus/gel-forming mucin-5AC|gastric mucin|lewis B blood group antigen|major airway glycoprotein|mucin 5, subtypes A and C, tracheobronchial/gastric|mucin 5AC, oligomeric mucus/gel-forming pseudogene|mucin-5 subtype AC, tracheobronchial|tracheobronchial mucin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MUC5AC, check out the MUC5AC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MUC5AC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal Antibody (M00612-1)
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No publications found for M00612-1
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Recommended Resources
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for nasal cavity epithelium using anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-27
Question
My question regarding product M00612-1, anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00612-1 anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-08
Question
Is a blocking peptide available for product anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody (M00612-1)?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
We do provide the blocking peptide for product anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody (M00612-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-25
Question
We are currently using anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody (M00612-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
Can we use M00612-1 antibody for human cells on WB and Immunofluorescence?
Verified customer
Asked: 2019-10-28
Answer
Yes. the Anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal Antibody M00612-1 antibody has been validated for Human samples and IF, IHC, ICC, and WB applications.
Boster Scientific Support
Answered: 2019-10-29
Question
Would M00612-1 anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
You can see on the product datasheet, M00612-1 anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-16
Question
We ordered your anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody for IF on colon in a previous experiment. I am using human, and We intend to use the antibody for WB next. I am interested in examining colon as well as tracheobronchial mucosa in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
I looked at the website and datasheets of our anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody and it seems that M00612-1 has been tested on human in both IF and WB. Thus M00612-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-08
Question
I see that the anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-27
Question
Is this M00612-1 anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody reactive to the isotypes of MUC5AC?
Z. Krishna
Verified customer
Asked: 2018-08-02
Answer
The immunogen of M00612-1 anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody is A synthesized peptide derived from human MUC5AC. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-02
Question
We have observed staining in human nasal cavity epithelium. Any tips? Is anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody supposed to stain nasal cavity epithelium positively?
K. Jha
Verified customer
Asked: 2018-07-31
Answer
According to literature nasal cavity epithelium does express MUC5AC. According to Uniprot.org, MUC5AC is expressed in nasal cavity epithelium, trachea, tracheobronchial mucosa, placenta trachea, nasal polyp, colon, stomach, among other tissues. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675
Boster Scientific Support
Answered: 2018-07-31
Question
I was wanting to use to test anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1 on human nasal cavity epithelium for research purposes, then I may be interested in using anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Parker
Verified customer
Asked: 2018-02-07
Answer
The products we sell, including anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-07
Question
I was wanting to use your anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody for WB for human nasal cavity epithelium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human nasal cavity epithelium identification?
O. Edwards
Verified customer
Asked: 2017-12-12
Answer
As indicated on the product datasheet, M00612-1 anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody has been tested for IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human nasal cavity epithelium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-12
Question
Is there a BSA free version of anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1 available?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-09
Question
Would anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1 work for WB with nasal cavity epithelium?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
According to the expression profile of nasal cavity epithelium, MUC5AC is highly expressed in nasal cavity epithelium. So, it is likely that anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1 will work for WB with nasal cavity epithelium.
Boster Scientific Support
Answered: 2017-08-24
Question
My colleagues were satisfied with the WB result of your anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody. However we have seen positive staining in nasal polyp secreted. using this antibody. Is that expected? Could you tell me where is MUC5AC supposed to be expressed?
K. Singh
Verified customer
Asked: 2017-08-03
Answer
From literature, nasal polyp does express MUC5AC. Generally MUC5AC expresses in secreted. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675
Boster Scientific Support
Answered: 2017-08-03
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for nasal cavity epithelium using anti-MUC5AC/Mucin 5Ac Rabbit Monoclonal antibody M00612-1. Let me know if you need anything else.
M. Wu
Verified customer
Asked: 2015-03-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-03-04
