Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1)

Product Name

Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1)

View all MUC5AC Antibodies

SKU/Catalog Number

MA1061

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) catalog # MA1061. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1061)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

45M1

Isotype

Mouse IgG1

Immunogen

Mucin from human ovarian cyst fluid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1061 is reactive to MUC5AC in Human, Rabbit, Rat

Applications

MA1061 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

585.57kDa

Background of MUC5AC

Mucin 5AC  (Muc5AC) is a protein that in humans is encoded by the MUC5AC gene. Using real-time RT-PCR, Moehle et al.  (2006) found that MUC5AC was highly expressed in adult trachea and stomach. Expression was lower in adult lung and weak in fetal lung, and no expression was detected in other tissues. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease. Moreover, Muc5ac is identified as a direct and critical mediator of resistance during intestinal nematode infection.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, rabbit, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, rabbit, rat, -
Western blot, 1-2μg/ml, Human, rabbit, rat

Validation Images & Assay Conditions

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Figure 1. IHC analysis of Mucin Gastric using anti-Mucin Gastric antibody (MA1061).
Mucin Gastric was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Mucin Gastric Antibody (MA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

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Figure 2. IHC analysis of Mucin Gastric using anti-Mucin Gastric antibody (MA1061).
Mucin Gastric was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Mucin Gastric Antibody (MA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Click image to see more details

Figure 3. IHC analysis of Mucin Gastric using anti-Mucin Gastric antibody (MA1061).
Mucin Gastric was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Mucin Gastric Antibody (MA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Gene/Protein Information For MUC5AC (Source: Uniprot.org, NCBI)

Alternative Names

gastric mucin; leB; lewis B blood group antigen; major airway glycoprotein; MUC5; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin 5AC, oligomeric mucus/gel-forming; mucin-5 subtype AC, tracheobronchial; mucin-5AC; TBM; tracheobronchial mucin MUC5AC MUC5, TBM, leB, mucin mucin 5AC, oligomeric mucus/gel-forming mucin-5AC|gastric mucin|lewis B blood group antigen|major airway glycoprotein|mucin 5, subtypes A and C, tracheobronchial/gastric|mucin 5AC, oligomeric mucus/gel-forming pseudogene|mucin-5 subtype AC, tracheobronchial|tracheobronchial mucin

For more info on MUC5AC, check out the MUC5AC Infographic

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In this infographic, you will see the following information for MUC5AC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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