Anti-P-GlycoProtein(MDR) ABCB1 Antibody (Monoclonal, F4)

Product Name

Anti-P-GlycoProtein(MDR) ABCB1 Antibody (Monoclonal, F4)

View all MDR1/ABCB1 Antibodies

SKU/Catalog Number

MA1060

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-P-GlycoProtein (MDR) ABCB1 Antibody (Monoclonal, F4) catalog # MA1060. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-P-GlycoProtein(MDR) ABCB1 Antibody (Monoclonal, F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1060)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

F4

Isotype

Mouse IgG1

Immunogen

Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1060 is reactive to ABCB1 in Human

Applications

MA1060 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

141.479kDa

Background of MDR1/ABCB1

P-Glycoprotein,, also known as Multidrug Resistance 1 (MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ABCB1 using anti-ABCB1 antibody (MA1060).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human DLD1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ABCB1 antigen affinity purified monoclonal antibody (Catalog # MA1060) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ABCB1 at approximately 200 kDa. The expected band size for ABCB1 is at 141 kDa.

Gene/Protein Information For ABCB1 (Source: Uniprot.org, NCBI)

Alternative Names

ABC20; ABCB1; ABCB1B; ATP-binding cassette sub-family B member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; CD243 antigen; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; EC 3.6.3; EC 3.6.3.44; GP170; IBD13; MDR1; MDR1MGC163296; multidrug resistance protein 1; P-glycoprotein 1; P-gp; PGY1; PGY1P-GP ABCB1 ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170 ATP binding cassette subfamily B member 1 ATP-dependent translocase ABCB1|ATP-binding cassette, sub-family B (MDR/TAP), member 1|P-glycoprotein 1|colchicin sensitivity|doxorubicin resistance|multidrug resistance protein 1|phospholipid transporter ABCB1

For more info on ABCB1, check out the ABCB1 Infographic

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In this infographic, you will see the following information for ABCB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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