Product Info Summary
SKU: | M00173-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p27 KIP 1 CDKN1B Rabbit Monoclonal Antibody
SKU/Catalog Number
M00173-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p27 KIP 1 CDKN1B Rabbit Monoclonal Antibody catalog # M00173-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p27 KIP 1 CDKN1B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00173-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CGA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p27 KIP 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00173-1 is reactive to CDKN1B in Human, Rat
Applications
M00173-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
29 kDa
Calculated molecular weight
22.073kDa
Background of p27/Kip1
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using p27 KIP 1 Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Immunofluorescent analysis of Hela cells, using p27 KIP 1 Antibody .
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Western blot analysis of p27 KIP 1 expression in MCF-7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDKN1B (Source: Uniprot.org, NCBI)
Gene Name
CDKN1B
Full Name
Cyclin-dependent kinase inhibitor 1B
Weight
22.073kDa
Superfamily
CDI family
Alternative Names
CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1 CDKN1B CDKN4, KIP1, MEN1B, MEN4, P27KIP1 cyclin dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B|cyclin-dependent kinase inhibitor 1B (p27, Kip1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKN1B, check out the CDKN1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKN1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p27 KIP 1 CDKN1B Rabbit Monoclonal Antibody (M00173-1)
Hello CJ!
M00173-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-p27 KIP 1 CDKN1B Rabbit Monoclonal Antibody
Question
Has M00173-1 been tested with IHC on prostate tissue?
Verified customer
Asked: 2020-12-23
Answer
Unfortunately, our lab hasn't tested the Anti-P27 KIP 1 CDKN1B Rabbit Monoclonal Antibody (M00173-1) with IHC on prostate tissue.
Boster Scientific Support
Answered: 2020-12-23