Anti-p53 Monoclonal Antibody (PAb122)

Product Name

Anti-p53 Monoclonal Antibody (PAb122)

View all p53 Antibodies

SKU/Catalog Number

M00001-4

Size

200µg

Form

Liquid

Description

Boster Bio Anti-p53 Monoclonal Antibody (PAb122) (Catalog# M00001-4). Tested in Flow Cytometry, ICC, IHC, IP, WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Cite This Product

Anti-p53 Monoclonal Antibody (PAb122) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00001-4)

Host

Mouse

Contents

Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Clonality

Monoclonal

Clone Number

pAb122

Isotype

IgG2b

Immunogen

SV40 transformed 3T3 cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Weakly cross-reacts with dog p53.

Reactive Species

M00001-4 is reactive to TP53 in Human, Mouse, Rat

Applications

M00001-4 is guaranteed for Flow Cytometry, IP, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

43.653kDa

Background of p53

Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1:250 colorimetric, 1:250 ECL). Detects a band of ~48/53kDa.
Immunohistochemistry (1:20 - 1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Tp53 using anti-Tp53 antibody (M00001-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tp53 antigen affinity purified polyclonal antibody (Catalog # M00001-4) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Tp53.

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Figure 2. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4).
Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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Figure 3. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4).
Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)

Alternative Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor antigen p53|antigen NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53

For more info on TP53, check out the TP53 Infographic

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In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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