Click image to see more details
-
-
-
-
-
+4
Product Info Summary
| SKU: | M00109-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC-P, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PDL1 CD274 Monoclonal Antibody [4F2]
SKU/Catalog Number
M00109-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PDL1 CD274 Monoclonal Antibody [4F2] (Catalog # M00109-2). Tested in ELISA, WB, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PDL1 CD274 Monoclonal Antibody [4F2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00109-2)
Host
Mouse
Contents
PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 4F2
Isotype
IgG1
Immunogen
PD-L1 antibody was raised against the extracellular domain of human PD-L1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.
Reactive Species
M00109-2 is reactive to CD274 in Human
Applications
M00109-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.275kDa
Background of PD-L1
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2). PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3). This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 μg/ml
Click image to see more details
Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Click image to see more details
Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 μg/mL.
Red: PDL1 Antibody [4F2] (RF16031)
Blue: DAPI staining
Click image to see more details
Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 μg/mL.
Red: PDL1 Antibody [4F2] (RF16031)
Blue: DAPI staining
Click image to see more details
Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 μg/mL.
Red: PDL1 Antibody [4F2] (M00109-2)
Blue: DAPI staining
Click image to see more details
Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 μg/mL.
Click image to see more details
Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 μg/mL.
Click image to see more details
Flow cytometry analysis of PD-L1 overexpressing HEK293 cells using PD-L1 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L1 overexpressing HEK293 cells.
Protein Target Info & Infographic
Gene/Protein Information For CD274 (Source: Uniprot.org, NCBI)
Gene Name
CD274
Full Name
Programmed cell death 1 ligand 1
Weight
33.275kDa
Superfamily
immunoglobulin superfamily
Alternative Names
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 antigen|PDCD1 ligand 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD274, check out the CD274 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD274: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDL1 CD274 Monoclonal Antibody [4F2] (M00109-2)
Hello CJ!
No publications found for M00109-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PDL1 CD274 Monoclonal Antibody [4F2]?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-PDL1 CD274 Monoclonal Antibody [4F2]
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
