Product Info Summary
SKU: | A01972T1402 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-MEK Kinase-1 (T1402) MAP3K1 Antibody
SKU/Catalog Number
A01972T1402
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-MEK Kinase-1 (T1402) MAP3K1 Antibody catalog # A01972T1402. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-MEK Kinase-1 (T1402) MAP3K1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01972T1402)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MEK Kinase-1 around the phosphorylation site of T1402.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01972T1402 is reactive to MAP3K1 in Human, Mouse, Rat
Applications
A01972T1402 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
164.47kDa
Background of MEKK1
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor. Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of MAP3K1 using Anti-Phospho-MEK Kinase-1 (T1402) MAP3K1 Antibody (A01972T1402).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°CClick image to see more details
Figure 1. Western blotting validation for Anti-Phospho-MEK Kinase-1 (T1402) MAP3K1 Antibody A01972T1402
Western Blot (WB) analysis of NIH-3T3 cells using Phospho-MEK Kinase-1 (T1402) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MAP3K1 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K1
Full Name
Mitogen-activated protein kinase kinase kinase 1
Weight
164.47kDa
Superfamily
protein kinase superfamily
Alternative Names
Map3k1; MAPK/ERK kinase kinase 1; MAPKKK1MAP/ERK kinase kinase 1; MEK kinase 1; MEKK1EC 2.7.11.25; MEKKMEKK 1; mitogen-activated protein kinase kinase kinase 1 MAP3K1 MAPKKK1, MEKK, MEKK 1, MEKK1, SRXY6 mitogen-activated protein kinase kinase kinase 1 mitogen-activated protein kinase kinase kinase 1|MAP/ERK kinase kinase 1|MAPK/ERK kinase kinase 1|MEK kinase 1|mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K1, check out the MAP3K1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-MEK Kinase-1 (T1402) MAP3K1 Antibody (A01972T1402)
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No publications found for A01972T1402
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