Anti-Phospho POLR2A (Ser1619) Antibody

Product Name

Anti-Phospho POLR2A (Ser1619) Antibody

View all RNA Polymerase II/POLR2A Antibodies

SKU/Catalog Number

P30431

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho POLR2A (Ser1619) Antibody (Catalog# P30431). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho POLR2A (Ser1619) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30431)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

BEF-20

Isotype

Rabbit IgG

Immunogen

A peptide derived from human POLR2A around the phosphorylation site of Ser1619. Immunogen sequence location: 1585-1634

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P30431 is reactive to POLR2A in Human, Monkey, Mouse, Rat

Applications

P30431 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

217.176kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.

Validation Images & Assay Conditions

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.

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Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.

Gene/Protein Information For POLR2A (Source: Uniprot.org, NCBI)

Alternative Names

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.48; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; POLR2; POLR2A; POLRA; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; RNA Pol 2; RNA Polymerase 2; RNA polymerase II subunit B1; RNA Polymerase II; RNA-directed RNA polymerase II subunit RPB1; RNAPII; RPB1; RPBh1; RpIILS; RPO2; RPOL2 POLR2A NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1|polymerase (RNA) II (DNA directed) polypeptide A, 220kDa|polymerase (RNA) II subunit A

For more info on POLR2A, check out the POLR2A Infographic

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In this infographic, you will see the following information for POLR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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