Anti-POLA2 Antibody Picoband™

Product Name

Anti-POLA2 Antibody Picoband™

View all Pola2 Antibodies

SKU/Catalog Number

A08427-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-POLA2 Antibody Picoband™ catalog # A08427-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-POLA2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08427-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human POLA2 recombinant protein (Position: N267-P579).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08427-2 is reactive to Pola2 in Human

Applications

A08427-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

66.214kDa

Background of Pola2

DNA polymerase alpha subunit 2 is an enzyme that in humans is encoded by the POLA2 gene. POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of POLA2 using anti-POLA2 antibody (A08427-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human COLO320 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLA2 antigen affinity purified polyclonal antibody (Catalog # A08427-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLA2 at approximately 70 kDa. The expected band size for POLA2 is at 66 kDa.

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Figure 2. Flow Cytometry analysis of U251 cells using anti-POLA2 antibody (A08427-2).
Overlay histogram showing U251 cells stained with A08427-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLA2 Antibody (A08427-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Pola2 (Source: Uniprot.org, NCBI)

Alternative Names

DNA polymerase alpha 70 kDa subunit; DNA polymerase alpha subunit B; FLJ21662; FLJ37250; polymerase (DNA directed), alpha 2 (70kD subunit); polymerase (DNA-directed), alpha (70kD) Pola2|AI573378|polymerase (DNA directed), alpha 2|DNA polymerase alpha subunit B|DNA polymerase alpha 2, 68 kDa|DNA polymerase alpha 70 kDa subunit

For more info on Pola2, check out the Pola2 Infographic

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In this infographic, you will see the following information for Pola2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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