Click image to see more details
Product Info Summary
| SKU: | A12720 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-POLR3F Antibody
SKU/Catalog Number
A12720
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-POLR3F Antibody (Catalog # A12720). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
POLR3F antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-POLR3F Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12720)
Host
Rabbit
Contents
POLR3F Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
POLR3F antibody was raised against a 21 amino acid synthetic peptide from near the amino terminus of human POLR3F. The immunogen is located within the first 50 amino acids of POLR3F.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
AP3M1 antibody may cross-react with AP3M2.
Reactive Species
A12720 is reactive to POLR3F in Human, Mouse, Rat
Applications
A12720 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
35.684kDa
Background of POLR3F
The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
POLR3F antibody can be used for detection of POLR3F by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of POLR3F in human brain tissue lysate with POLR3F antibody at (A) 0.5 and (B) 1 μg/mL.
Click image to see more details
Immunohistochemistry of POLR3F in human brain tissue with POLR3F antibody at 2.5 μg/mL.
Click image to see more details
Immunofluorescence of POLR3F in human brain tissue with POLR3F antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For POLR3F (Source: Uniprot.org, NCBI)
Gene Name
POLR3F
Full Name
DNA-directed RNA polymerase III subunit RPC6
Weight
35.684kDa
Superfamily
eukaryotic RPC34/RPC39 RNA polymerase subunit family
Alternative Names
DNA-directed RNA polymerase III 39 kDa polypeptide; DNA-directed RNA polymerase III subunit F; DNA-directed RNA polymerase III subunit RPC6; DNA-directed RNA polymerases III 39 kDa polypeptide; polymerase (RNA) III (DNA directed) polypeptide F (39 kDa); polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa; RNA polymerase III 39 kDa subunit; RNA polymerase III C39 subunit; RNA polymerase III subunit C6; RPC39MGC13517; RPC6 POLR3F C34, RPC39, RPC6 RNA polymerase III subunit F DNA-directed RNA polymerase III subunit RPC6|RNA polymerase III C39 subunit|RNA polymerase III subunit C6|polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa|polymerase (RNA) III subunit F
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR3F, check out the POLR3F Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR3F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLR3F Antibody (A12720)
Hello CJ!
No publications found for A12720
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-POLR3F Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-POLR3F Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
