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Product Info Summary
| SKU: | A00313-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Polycomb complex protein BMI-1 Bmi1 Antibody
SKU/Catalog Number
A00313-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Polycomb complex protein BMI-1 Bmi1 Antibody catalog # A00313-1. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Polycomb complex protein BMI-1 Bmi1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00313-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CHOP around the phosphorylation site of S30.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00313-1 is reactive to BMI1 in Human, Rat
Applications
A00313-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.949kDa
Background of BMI-1
HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of BMI1 using Anti-Polycomb complex protein BMI-1 Bmi1 Antibody (A00313-1).
ICC staining Bmi1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Polycomb complex protein BMI-1 Bmi1 Antibody A00313-1
Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For BMI1 (Source: Uniprot.org, NCBI)
Gene Name
BMI1
Full Name
Polycomb complex protein BMI-1
Weight
36.949kDa
Alternative Names
B lymphoma Mo-MLV insertion region 1 homolog (mouse); B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1; BMI-1; FLVI2/BMI1; MGC12685; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; RING finger protein 51; RNF51; RNF51Polycomb group RING finger protein 4 BMI1 FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1 BMI1 proto-oncogene, polycomb ring finger polycomb complex protein BMI-1|B lymphoma Mo-MLV insertion region 1 homolog|BMI1 polycomb ring finger oncogene|BMI1 polycomb ring finger proto-oncogene|murine leukemia viral (bmi-1) oncogene homolog|polycomb group RING finger protein 4|polycomb group protein Bmi1|ring finger protein 51
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BMI1, check out the BMI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BMI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Polycomb complex protein BMI-1 Bmi1 Antibody (A00313-1)
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No publications found for A00313-1
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