Anti-Prokineticin 1/PROK1 Antibody Picoband™

Product Name

Anti-Prokineticin 1/PROK1 Antibody Picoband™

View all EG-VEGF/PK1 Antibodies

SKU/Catalog Number

PB9882

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Prokineticin 1/PROK1 Antibody Picoband™ catalog # PB9882. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Prokineticin 1/PROK1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9882)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse Prokineticin 1 recombinant protein (Position: A20-F105). Mouse Prokineticin 1 shares 88.4% and 94.2% amino acid (aa) sequence identity with human and rat Prokineticin 1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9882 is reactive to PROK1 in Mouse

Applications

PB9882 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

12 kDa

Calculated molecular weight

11.715kDa

Background of EG-VEGF/PK1

Prokineticin-1 is also known as EG-VEGF. The International Radiation Hybrid Mapping Consortium mapped the PROK1 gene to chromosome 1. The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Prokineticin 1 using anti-Prokineticin 1 antibody (PB9882).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prokineticin 1 antigen affinity purified polyclonal antibody (Catalog # PB9882) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prokineticin 1 at approximately 12 kDa. The expected band size for Prokineticin 1 is at 12 kDa.

Gene/Protein Information For PROK1 (Source: Uniprot.org, NCBI)

Alternative Names

EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1 PROK1 EGVEGF, PK1, PRK1 prokineticin 1 prokineticin-1|EG-VEGF|black mamba toxin-related protein|endocrine-gland-derived vascular endothelial growth factor|mambakine

For more info on PROK1, check out the PROK1 Infographic

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In this infographic, you will see the following information for PROK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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