Anti-PTEN-P1 PTH2 Antibody

PTEN antibody

Boster Bio Anti-PTEN-P1 PTH2 Antibody (Catalog # A08380). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A08380
Size: 100ug
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-PTEN-P1 PTH2 Antibody

View all PTEN Antibodies

SKU/Catalog Number

A08380

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-PTEN-P1 PTH2 Antibody (Catalog # A08380). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-PTEN-P1 PTH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08380)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08380 is reactive to PTEN in Human

Applications

A08380 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

47.166kDa

Background of PTEN

This gene (PTENP1) is a highly homologous pseudogene of PTEN. PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tension like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:160,000
WB: 1:500 to 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For PTEN (Source: Uniprot.org, NCBI)

Gene Name

PTEN

Full Name

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

Weight

47.166kDa

Superfamily

PTEN phosphatase protein family

Alternative Names

BZS; EC 3.1.3.16,10q23del; EC 3.1.3.48; EC 3.1.3.67; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; MMAC1; MMAC1MGC11227; Mutated in multiple advanced cancers 1; phosphatase and tensin homologDEC; phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN; PTEN; PTEN1; TEP1MHAM PTEN 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, PTENbeta, TEP1, PTEN phosphatase and tensin homolog phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|MMAC1 phosphatase and tensin homolog deleted on chromosome 10|PTENepsilon|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|protein tyrosine phosphatase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PTEN, check out the PTEN Infographic

PTEN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-PTEN-P1 PTH2 Antibody

Question

Is a blocking peptide available for product anti-PTEN-P1 antibody (A08380)?

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

We do provide the blocking peptide for product anti-PTEN-P1 antibody (A08380). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-07

Question

Is there a BSA free version of anti-PTEN-P1 antibody A08380 available?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PTEN-P1 antibody A08380 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-17

Question

We are currently using anti-PTEN-P1 antibody A08380 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

The anti-PTEN-P1 antibody (A08380) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-05

Question

My question regarding product A08380, anti-PTEN-P1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08380 anti-PTEN-P1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-29

Question

Will anti-PTEN-P1 antibody A08380 work on pig WB with cerebellar hemisphere?

Verified Customer

Verified customer

Asked: 2018-10-16

Answer

Our lab technicians have not tested anti-PTEN-P1 antibody A08380 on pig. You can run a BLAST between pig and the immunogen sequence of anti-PTEN-P1 antibody A08380 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cerebellar hemisphere in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-16

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-PTEN-P1 antibody A08380. Let me know if you need anything else.

L. Miller

Verified customer

Asked: 2017-10-31

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-31

Question

I see that the anti-PTEN-P1 antibody A08380 works with WB, what is the protocol used to produce the result images on the product page?

M. Mitchell

Verified customer

Asked: 2013-11-04

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-11-04

Order DetailsPrice
A08380-100uL

100uL

$599

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A08380
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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