Anti-Secretogranin 3/SCG3 Antibody

Product Name

Anti-Secretogranin 3/SCG3 Antibody

View all Secretogranin 3 Antibodies

SKU/Catalog Number

PA1071

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Secretogranin 3/SCG3 Antibody catalog # PA1071. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Secretogranin 3/SCG3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1071)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Secretogranin 3, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1071 is reactive to Scg3 in Human, Mouse, Rat

Applications

PA1071 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

62-65 kDa

Calculated molecular weight

53.326kDa

Background of Secretogranin 3

SCG3 (secretogranin III) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Genetic variations in the SCG3 gene may influence the risk of obesity through possible regulation of hypothalamic neuropeptide secretion. SCG3 was the only gene within a haplotype block that contained rs3764220. SCG3 mRNA and immunoreactivity were detected in the paraventricular nucleus, lateral hypothalamic area, and arcuate nucleus, and the protein coexisted with orexin, melanin-concentrating hormone, neuropeptide Y, and proopiomelanocortin. SCG3 formed a granule-like structure together with these neuropeptides.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

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Anti-Secretogranin 3 antibody, PA1071, Western blotting
WB: HELA Cell Lysate

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Anti-Secretogranin 3 antibody, PA1071, IHC(P)
IHC(P): Rat Brain Tissue

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Anti-Secretogranin 3 antibody, PA1071, IHC(P)
IHC(P): Human Rectal Cancer Tissue

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Figure 4.Western blot analysis of SCG3 using anti-SCG3antibody (PA1071).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Marker 1113,
Lane 2: rat brain tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat PC-12 whole cell lysates.
Lane 5: mouse brain tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse pancreas tissue lysates,
Lane 8: mouse NEURO-2A whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCG3 antigen affinity purified polyclonal antibody (Catalog # PA1071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCG3 at approximately 62-65KD. The expected band size for SCG3 is at 53KD.

Gene/Protein Information For Scg3 (Source: Uniprot.org, NCBI)

Alternative Names

SCG3; Secretogranin III; secretogranin IIIFLJ90833; Secretogranin-3; SGIII Scg3|1B1075, AI385542, Chgd, S, SgIII|secretogranin III|secretogranin-3

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In this infographic, you will see the following information for Scg3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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