Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband™

Product Name

Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband™

View all GAT-1/SLC6A1 Antibodies

SKU/Catalog Number

A05109

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband™ catalog # A05109. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05109)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A1, different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05109 is reactive to SLC6A1 in Human, Mouse, Rat

Applications

A05109 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

88 kDa

Calculated molecular weight

67.074kDa

Background of GAT-1/SLC6A1

GABA transporter 1 (GAT1), also known as sodium- and chloride-dependent GABA transporter 1, is a protein that in humans is encoded by the SLC6A1 gene. GABA Transporter 1 uses Na+ and Cl- to create a gradient, which removes or adds GABA to extracellular spaces in the cerebrum and cerebellum. The stoichiometry for GABA Transporter 1 is 2 Na+: 1 Cl-: 1 GABA. The activity of GAT1 is largely dependent on the presence of Na+, while Cl- assists by increasing the ability for GAT-1 to uptake GABA.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SLC6A1 using anti-SLC6A1 antibody (A05109).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A1 antigen affinity purified polyclonal antibody (Catalog # A05109) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A1 at approximately 88KD. The expected band size for SLC6A1 is at 67KD.

Gene/Protein Information For SLC6A1 (Source: Uniprot.org, NCBI)

Alternative Names

GABA Transporter 1; GABATHG; GABATR; GABATRGAT-1; GABT1; GAT1; GAT-1; GAT1sodium- and chloride-dependent GABA transporter 1; SLC6A1; solute carrier family 6 (neurotransmitter transporter, GABA), member 1; Solute carrier family 6 member 1 SLC6A1 GABATHG, GABATR, GAT1, MAE solute carrier family 6 member 1 sodium- and chloride-dependent GABA transporter 1|GABA transporter 1|solute carrier family 6 (neurotransmitter transporter), member 1|solute carrier family 6 (neurotransmitter transporter, GABA), member 1

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