Anti-SMAD3-S208 Antibody

Product Name

Anti-SMAD3-S208 Antibody

View all SMAD3 Antibodies

SKU/Catalog Number

A00059

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-SMAD3-S208 Antibody (Catalog # A00059). Tested in WB, Flow Cytometry, IF, IHC-P application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-SMAD3-S208 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00059)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from human SMAD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00059 is reactive to SMAD3 in Human, Mouse

Applications

A00059 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

48.081kDa

Background of SMAD3

SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50

Validation Images & Assay Conditions

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Confocal immunofluorescent analysis of SMAD3-S208 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

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SMAD3 Antibody (S208) (Cat. #A00059) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane). This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).

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SMAD3-S208 Antibody (cat. #A00059) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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SMAD3-S208 Antibody (Cat. #A00059) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Gene/Protein Information For SMAD3 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; HsT17436; JV15-2MGC60396; MAD homolog 3; mad protein homolog; MAD, mothers against decapentaplegic homolog 3 (Drosophila); MAD, mothers against decapentaplegic homolog 3; mad3; MADH3mad homolog JV15-2; mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD 3; SMAD family member 3HSPC193; SMAD, mothers against DPP homolog 3 (Drosophila); SMAD, mothers against DPP homolog 3; Smad3 SMAD3 HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3 SMAD family member 3 mothers against decapentaplegic homolog 3|MAD homolog 3|MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|hMAD-3|hSMAD3|mad homolog JV15-2|mad protein homolog|mad3|mothers against DPP homolog 3

For more info on SMAD3, check out the SMAD3 Infographic

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In this infographic, you will see the following information for SMAD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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