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Product Info Summary
| SKU: | A00752-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Smpd1 Antibody Picoband™
SKU/Catalog Number
A00752-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Smpd1 Antibody Picoband™ catalog # A00752-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Smpd1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00752-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Smpd1 recombinant protein (Position: D204-M558).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00752-1 is reactive to SMPD1 in Mouse, Rat
Applications
A00752-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
69.936kDa
Background of SMPD1
Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), is an enzyme that in humans is encoded by the SMPD1 gene. Enables acid sphingomyelin phosphodiesterase activity and zinc ion binding activity. Involved in ceramide biosynthetic process; positive regulation of apoptotic process; and response to ionizing radiation. Acts upstream of or within ceramide metabolic process; cholesterol metabolic process; and sphingomyelin catabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; integumental system; nervous system; sensory organ; and skeleton. Used to study Niemann-Pick disease. Human ortholog(s) of this gene implicated in Niemann-Pick disease; Niemann-Pick disease type A; and Niemann-Pick disease type B. Orthologous to human SMPD1 (sphingomyelin phosphodiesterase 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Smpd1 using anti-Smpd1 antibody (A00752-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smpd1 antigen affinity purified polyclonal antibody (Catalog # A00752-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smpd1 at approximately 70 kDa. The expected band size for Smpd1 is at 70 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SMPD1 (Source: Uniprot.org, NCBI)
Gene Name
SMPD1
Full Name
Sphingomyelin phosphodiesterase
Weight
69.936kDa
Superfamily
acid sphingomyelinase family
Alternative Names
Acid sphingomyelinase; aSMase; ASMsphingomyelin phosphodiesterase; EC 3.1.4.12; NPD; SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal SMPD1 ASM, ASMASE, NPD sphingomyelin phosphodiesterase 1 sphingomyelin phosphodiesterase|Niemann-Pick type A/B|acid sphingomyelinase|sphingomyelin phosphodiesterase 1, acid lysosomal
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMPD1, check out the SMPD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMPD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smpd1 Antibody Picoband™ (A00752-1)
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