Product Info Summary
SKU: | P01625 |
---|---|
Size: | 100 µl |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-SNAP25 (Ser187) Antibody
SKU/Catalog Number
P01625
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-SNAP25 (Ser187) Antibody (Catalog # P01625). Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-SNAP25 (Ser187) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01625)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Clone Number
608
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 of rat SNAP25, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01625 is reactive to SNAP25 in Mouse, Rat
Applications
P01625 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
23.315kDa
Background of SNAP25
SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer’s Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on Ser-187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since Ser-187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25 kDa SNAP25 phosphorylated at Ser187 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (gamma-Ptase , 400 units/100ul lysate for 30 minutes, RT).
Protein Target Info & Infographic
Gene/Protein Information For SNAP25 (Source: Uniprot.org, NCBI)
Gene Name
SNAP25
Full Name
Synaptosomal-associated protein 25
Weight
23.315kDa
Superfamily
SNAP-25 family
Alternative Names
bA416N4.2; dJ1068F16.2; FLJ23079; resistance to inhibitors of cholinesterase 4 homolog; RIC4; RIC-4; SEC9; SNAP25; SNAP-25SNAP; SUP; Super protein; Synaptosomal-associated 25 kDa protein; synaptosomal-associated protein 25; synaptosomal-associated protein, 25kD; synaptosomal-associated protein, 25kDa SNAP25 CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2 synaptosome associated protein 25 synaptosomal-associated protein 25|resistance to inhibitors of cholinesterase 4 homolog|synaptosomal-associated protein, 25kDa|synaptosome associated protein 25kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNAP25, check out the SNAP25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNAP25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-SNAP25 (Ser187) Antibody (P01625)
Hello CJ!
P01625 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Guan Y,Chen X,Zhao B,Shi Y,Han F.What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.Cell Mol Neurobiol.2020 Sep 15.doi:10.1007/s10571-020-00960-w.Epub ahead of print.PMID:32930942.
Species: Rat
Zhong ZQ, Xiang Y, Hu X, Wang YC, Zeng X, Wang XM, Xia QJ, Wang TH, Zhang X. Neural Regen Res. 2017 Jun;12(6):969-976. doi: 10.4103/1673-5374.208592. Synaptosomal-associated protein 25 may be an intervention target for improving sensory and locomo...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-SNAP25 (Ser187) Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Phospho-SNAP25 (Ser187) Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Phospho-SNAP25 (Ser187) Antibody
Question
We have observed staining in rat brain. Are there any suggestions? Is anti-Phospho-SNAP25 (Ser187) antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
According to literature brain does express SNAP25. According to Uniprot.org, SNAP25 is expressed in frontal cortex, fetal brain temporal cortex, skeletal muscle, amygdala hippocampus, brain, eye, among other tissues. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2019-11-19
Question
We are currently using anti-Phospho-SNAP25 (Ser187) antibody P01625 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on primate tissues as well?
T. Krishna
Verified customer
Asked: 2019-09-02
Answer
The anti-Phospho-SNAP25 (Ser187) antibody (P01625) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-02
Question
Our team were satisfied with the WB result of your anti-Phospho-SNAP25 (Ser187) antibody. However we have observed positive staining in fetal brain temporal cortex perinuclear region using this antibody. Is that expected? Could you tell me where is SNAP25 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
According to literature, fetal brain temporal cortex does express SNAP25. Generally SNAP25 expresses in cytoplasm, perinuclear region. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2017-05-22