Anti-SP1 Rabbit Monoclonal Antibody

Product Name

Anti-SP1 Rabbit Monoclonal Antibody

View all SP1 Antibodies

SKU/Catalog Number

M00110

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-SP1 Rabbit Monoclonal Antibody catalog # M00110. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00110)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOOC-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human SP1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00110 is reactive to SP1 in Human

Applications

M00110 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

63 kDa

Calculated molecular weight

80.693kDa

Background of SP1

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Western blot analysis of SP1 expression in HeLa cell lysate.

Gene/Protein Information For SP1 (Source: Uniprot.org, NCBI)

Alternative Names

Sp1 transcription factor; specificity protein 1; transcription factor Sp1; TSFP1 SP1 Sp1 transcription factor transcription factor Sp1|specificity protein 1

For more info on SP1, check out the SP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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