Product Info Summary
SKU: | A00674S616 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-TCF-4/12 (S616) Antibody
SKU/Catalog Number
A00674S616
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TCF-4/12 (S616) Antibody catalog # A00674S616. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCF-4/12 (S616) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00674S616)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human TCF-4/12
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00674S616 is reactive to TCF4 in Human, Mouse, Rat
Applications
A00674S616 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
71.308kDa
Background of TCF4
Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription By similarity. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
Corneliussen B., J. Virol. 65:6084-6093(1991).
Breschel T.S., Hum. Mol. Genet. 6:1855-1863(1997).
Amiel J., Am. J. Hum. Genet. 80:988-993(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-TCF-4/12 (S616) Antibody A00674S616
Western blot (WB) analysis of TCF-4/12 (S616) pAb at 1:500 dilutionLane1:SGC7901 whole cell lysate(20ug)
Lane2:HepG2 whole cell lysate(40ug)
Lane3:Jurkat whole cell lysate(40ug)
Lane4:A549 whole cell lysate(40ug)
Lane5:PMVEC whole cell lysate(40ug)
Lane6:AML-12 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For TCF4 (Source: Uniprot.org, NCBI)
Gene Name
TCF4
Full Name
Transcription factor 4
Weight
71.308kDa
Alternative Names
BHLHB19; bHLHb19E2-2; Class B basic helix-loop-helix protein 19; Immunoglobulin transcription factor 2; ITF-2; ITF2PTHS; MGC149724; SEF-2; SEF2-1; SEF2-1A; SEF2-1B; SEF2MGC149723; SL3-3 enhancer factor 2; TCF-4; transcription factor 4 TCF4 CDG2T, E2-2, FECD3, ITF-2, ITF2, PTHS, SEF-2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, SEF2-1D, TCF-4, bHLHb19 transcription factor 4 transcription factor 4|SL3-3 enhancer factor 2|class B basic helix-loop-helix protein 19|immunoglobulin transcription factor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TCF4, check out the TCF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TCF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TCF-4/12 (S616) Antibody (A00674S616)
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No publications found for A00674S616
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