Anti-TIM-1 HAVCR1 Antibody

Product Name

Anti-TIM-1 HAVCR1 Antibody

View all TIM-1/KIM-1/HAVCR Antibodies

SKU/Catalog Number

A01306-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TIM-1 HAVCR1 Antibody (Catalog # A01306-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

TIM-1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TIM-1 HAVCR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01306-1)

Host

Rabbit

Contents

TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-TIM-1 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-1. The immunogen is located within amino acids 50 - 100 of TIM-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

FLIP recognizes all FLIP splice variants including FLIPa, b, and y.

Reactive Species

A01306-1 is reactive to HAVCR1 in Human, Mouse

Applications

A01306-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

39.25kDa

Background of TIM-1/KIM-1/HAVCR

The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1 - 8 μg/mL (overnight incubation at 4˚ C); IHC-P: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: TIM-1 A01306-1 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: TIM-1 A01306-1 (8 μg/mL), TIM-1 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C or 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Validation with TIM-1 siRNA Knockdown in Hela Cells
HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 μg of HeLa whole cell lysates per lane. Antibodies: A01306-1 (8 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in the Mouse Cell Line
Loading: 15 μg of NIH/3T3 cell lysates. Antibodies: TIM-1 A01306-1 (8 μg/mL)

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Immunofluorescence Validation of TIM-1 in Human Uterus Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1 with A01306-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.

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Immunohistochemistry Validation of TIM-1 in Human Uterus Tissue
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (A01306-1) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Gene/Protein Information For HAVCR1 (Source: Uniprot.org, NCBI)

Alternative Names

CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1 HAVCR1 CD365, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1 hepatitis A virus cellular receptor 1 hepatitis A virus cellular receptor 1|T cell immunoglobin domain and mucin domain protein 1|T-cell immunoglobulin mucin family member 1|T-cell immunoglobulin mucin receptor 1|T-cell membrane protein 1|kidney injury molecule 1

For more info on HAVCR1, check out the HAVCR1 Infographic

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In this infographic, you will see the following information for HAVCR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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