Anti-TIMP-4 (S206) Antibody

Product Name

Anti-TIMP-4 (S206) Antibody

View all TIMP-4 Antibodies

SKU/Catalog Number

A07131S206

Size

100ul

Form

Liquid

Description

Boster Bio Anti-TIMP-4 (S206) Antibody catalog # A07131S206. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TIMP-4 (S206) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07131S206)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human TAF3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07131S206 is reactive to TIMP4 in Human, Mouse, Rat

Applications

A07131S206 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

25.503kDa

Background of TIMP-4

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

Gangloff Y.G., Mol. Cell. Biol. 21:5109-5121(2001) [PubMed: 11438666].
Deloukas P., Nature 429:375-381(2004) [PubMed: 15164054].
Bechtel S., BMC Genomics 8:399-399(2007) [PubMed: 17974005].

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of TIMP4 using Anti-TIMP-4 (S206) Antibody (A07131S206).

Immunohistochemistry (IHC) analyzes of TIMP-4 (S206) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-TIMP-4 (S206) Antibody A07131S206

Western blot (WB) analysis of TIMP-4 (S206) polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:H9C2 whole cell lysate(40ug)
Lane3:The Heart tissue lysate of Rat(40ug)
Lane4:The Heart tissue lysate of Mouse(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Click image to see more details

Figure 3. Western blotting validation for Anti-TIMP-4 (S206) Antibody A07131S206

Western blot (WB) analysis of TIMP-4 (S206) pAb at 1:500 dilution
Lane1:A549 whole cell lysate(40ug)
Lane2::L02 whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Lane4:The Heart tissue lysate of Mouse(40ug)
Lane5:The Heart tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For TIMP4 (Source: Uniprot.org, NCBI)

Alternative Names

metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4 TIMP4 TIMP-4 TIMP metallopeptidase inhibitor 4 metalloproteinase inhibitor 4|tissue inhibitor of metalloproteinase 4|tissue inhibitor of metalloproteinases 4

For more info on TIMP4, check out the TIMP4 Infographic

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In this infographic, you will see the following information for TIMP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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