Anti-TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Monoclonal Antibody

Product Name

Anti-TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Monoclonal Antibody

View all TIMP-2 Antibodies

SKU/Catalog Number

M01037

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Monoclonal Antibody (Catalog # M01037). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Guinea pig, Rabbit, Cow, Zebrafish.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01037)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM356

Isotype

IgG2a, kappa

Immunogen

A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01037 is reactive to TIMP2 in Cow, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

M01037 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

24.399kDa

Background of TIMP-2

This monoclonal antibody recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Placenta stained with Anti-TIMP2 Monoclonal Antibody (SPM356).

Gene/Protein Information For TIMP2 (Source: Uniprot.org, NCBI)

Alternative Names

CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2 TIMP2 CSC-21K, DDC8 TIMP metallopeptidase inhibitor 2 metalloproteinase inhibitor 2|testicular secretory protein Li 57|tissue inhibitor of metalloproteinases 2

For more info on TIMP2, check out the TIMP2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TIMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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