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Product Info Summary
| SKU: | A00207-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Transforming protein RhoA RhoA Antibody
SKU/Catalog Number
A00207-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Transforming protein RhoA RhoA Antibody catalog # A00207-2. Tested in WB,ICC/IF,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transforming protein RhoA RhoA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00207-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CREB-BP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00207-2 is reactive to RHOA in Human, Mouse, Rat
Applications
A00207-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21.768kDa
Background of RHOA
Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers.
Zoi Lygerou, Nucleic Acids Res., Feb 1994; 22: 5332 - 5340.
Karsten Grote, J. Biol. Chem., Dec 2004; 279: 55675 - 55681.
Kumi Nagamoto-Combs, J. Biol. Chem., Feb 1997; 272: 6051.
Stephen J. Elliman, Isaac, J. Biol. Chem., Jan 2006; 281: 16 -19
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of RhoA on rat brain lysates using anti-RhoA antibody at 1/1,000 dilution.
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ICC staining RhoA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Protein Target Info & Infographic
Gene/Protein Information For RHOA (Source: Uniprot.org, NCBI)
Gene Name
RHOA
Full Name
Transforming protein RhoA
Weight
21.768kDa
Superfamily
small GTPase superfamily
Alternative Names
h12; oncogene RHO H12; ras homolog gene family, member A; Rho cDNA clone 12; RHO12; RhoA; RHOH12ARH12ARHAAplysia ras-related homolog 12; small GTP binding protein RhoA; transforming protein RhoA RHOA ARH12, ARHA, EDFAOB, RHO12, RHOH12 ras homolog family member A transforming protein RhoA|Aplysia ras-related homolog 12|epididymis secretory sperm binding protein|oncogene RHO H12|small GTP binding protein RhoA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHOA, check out the RHOA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHOA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transforming protein RhoA RhoA Antibody (A00207-2)
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No publications found for A00207-2
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Customer Reviews
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