Anti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31)

Product Name

Anti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31)

View all Tropomyosin-1 Antibodies

SKU/Catalog Number

MA1095

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Tropomyosin (36/39 kDa) Tpm1 Antibody (Monoclonal, TM31) catalog # MA1095. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1095)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

TM31

Isotype

Mouse IgG1

Immunogen

Chicken gizzard tropomyosin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1095 is reactive to TPM1 in Chicken, Human, Mouse, Rat

Applications

MA1095 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

35-45 kDa

Calculated molecular weight

32.709kDa

Background of Tropomyosin-1

The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.5-1μg/ml, Human, mouse, rat, chicken

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Tropomyosin(36/39kDa) using anti-Tropomyosin(36/39kDa) antibody (MA1095).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tropomyosin(36/39kDa) antigen affinity purified monoclonal antibody (Catalog # MA1095) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tropomyosin(36/39kDa) at approximately 35-45 kDa. The expected band size for Tropomyosin(36/39kDa) is at 33 kDa.

Gene/Protein Information For TPM1 (Source: Uniprot.org, NCBI)

Alternative Names

alpha tropomyosin; alpha-tropomyosin; C15orf13; cardiomyopathy, hypertrophic 3; chromosome 15 open reading frame 13; CMD1Y; CMH3; HTM-alpha; sarcomeric tropomyosin kappa; TMSA; tropomyosin 1 (alpha) isoform 1; tropomyosin 1 (alpha) isoform 2; tropomyosin 1 (alpha) isoform 3; tropomyosin 1 (alpha) isoform 4; tropomyosin 1 (alpha) isoform 5; tropomyosin 1 (alpha) isoform 6; tropomyosin 1 (alpha) isoform 7; tropomyosin 1 (alpha); tropomyosin alpha-1 chain; tropomyosin-1 TPM1 C15orf13, CMD1Y, CMH3, HEL-S-265, HTM-alpha, LVNC9, TMSA tropomyosin 1 tropomyosin alpha-1 chain|cardiomyopathy, hypertrophic 3|epididymis secretory protein Li 265|sarcomeric tropomyosin kappa

For more info on TPM1, check out the TPM1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TPM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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