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Product Info Summary
| SKU: | M01409 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TXNIP Monoclonal Antibody
SKU/Catalog Number
M01409
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TXNIP Monoclonal Antibody catalog # M01409. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TXNIP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01409)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEBA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TXNIP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01409 is reactive to TXNIP in Human, Mouse, Rat
Applications
M01409 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
43.661kDa
Background of TXNIP
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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Western blot analysis of TXNIP expression in BxPC-3 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TXNIP (Source: Uniprot.org, NCBI)
Gene Name
TXNIP
Full Name
Thioredoxin-interacting protein
Weight
43.661kDa
Superfamily
arrestin family
Alternative Names
HHCPA78; THIF; thioredoxin binding protein 2; thioredoxin interacting protein; Thioredoxin-binding protein 2; thioredoxin-interacting protein; upregulated by 1,25-dihydroxyvitamin D-3; VDUP1EST01027; Vitamin D3 up-regulated protein 1 TXNIP ARRDC6, EST01027, HHCPA78, THIF, VDUP1 thioredoxin interacting protein thioredoxin-interacting protein|thioredoxin binding protein 2|upregulated by 1,25-dihydroxyvitamin D-3|vitamin D3 up-regulated protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TXNIP, check out the TXNIP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TXNIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TXNIP Monoclonal Antibody (M01409)
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No publications found for M01409
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-TXNIP Monoclonal Antibody
Question
Does M01409 anti-TXNIP Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
As indicated on the product datasheet, M01409 anti-TXNIP Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-09
Question
What is the sample target on Western blot analysis in your product image for M01409?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-TXNIP Monoclonal Antibody (M01409) expression in BxPC-3 cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
I have a question about product M01409, anti-TXNIP Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01409 anti-TXNIP Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-10
Question
Is a blocking peptide available for product anti-TXNIP Monoclonal antibody (M01409)?
J. Zhang
Verified customer
Asked: 2015-11-30
Answer
We do provide the blocking peptide for product anti-TXNIP Monoclonal antibody (M01409). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-11-30
Question
We are currently using anti-TXNIP Monoclonal antibody M01409 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
A. Jones
Verified customer
Asked: 2013-08-13
Answer
The anti-TXNIP Monoclonal antibody (M01409) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-13
