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- Table of Contents
Autophagy protein 5 Antibodies
AND ATG5 ELISA kits, Autophagy protein 5 Proteins
Many biological assays use antibodies to detect Autophagy protein 5, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Autophagy protein 5 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Autophagy protein 5 antibodies...
Designing a Western blot for Autophagy protein 5? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ATG5 Western Blot GuideDesigning an IHC experiment for Autophagy protein 5? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the ATG5 IHC GuideATG5 Infographic by Boster Bio
All ATG5 Products
Product
Figures
Specificity
Applications
Price
Anti-APG5L/ATG5 Antibody Picoband®
14 Citations
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # PA2260
100 μg/vial
Cat # PA2260
100 μg/vial
Anti-ATG5/Apg5 Rabbit Monoclonal Antibody
14 Citations
Human, Mouse, Rat
IP, IF, IHC, ICC, WB
$370
Cat # M00240
100 μl
Cat # M00240
100 μl
Human, Mouse, Rat
ELISA, IF, IHC-P, WB
$481
Cat # A00240
0.1 mg
Cat # A00240
0.1 mg
Human, Mouse, Rat
ELISA, IP, IF, IHC, WB
$146
Cat # M00240-2
20μL, 40μL, 100μL, 200μL
Cat # M00240-2
20μL, 40μL, 100μL, 200μL
ATG5 Overview
Facts about Autophagy protein 5.
Autophagy protein 5 (ATG5)
Conjugation with ATG12, through a ubiquitin-like conjugating system between ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is crucial for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme that's needed for lipidation of ATG8 family proteins and their association to the vesicle membranes.
Involved in mitochondrial quality control after oxidative damage, and in following cellular longevity. Plays a critical role in a number of facets of lymphocyte development and is critical for both B and T lymphocyte survival and proliferation.
Gene Information
| Human | |
|---|---|
| Gene Name: | ATG5 |
| Uniprot: | Q9H1Y0 |
| Entrez: | 9474 |
Family
| Belongs to: |
|---|
| ATG5 family |
Alternative Names
APG5 autophagy 5-like (S. cerevisiae); APG5; APG5LS. cerevisiae)-like; ASP; ASPAPG5-LIKE; ATG5 autophagy related 5 homolog (S. cerevisiae); ATG5; Autophagy protein 5
Molecular Weight
Mass (kDA):
32.447 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 6q21 |
| Sequence: | 6; NC_000006.12 (106184476..106325820, complement) |
Tissue Specificity
Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Subcellular Localizations
Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Diseases
- Malignant Neoplasms
- Neoplasms
- Carcinoma
- Nerve Degeneration
- Cell Transformation, Neoplastic
- Virus Diseases
- Neurodegenerative Disorders
- Melanoma
- Glioma
- Hypoxia
- Malignant Paraganglionic Neoplasm
- Malignant Neoplasm Of Breast
Interacting Proteins
- ATG16L1
- CCDC12
- GABARAP
- MAP1LC3B
- RB1CC1
- TECPR1
- WDFY3
Pathways
- Autophagy
- Cell Death
- Macroautophagy
- Autophagic Cell Death
- Pathogenesis
- Conjugation
- Programmed Cell Death
- Cell Cycle
- Senescence
- Cell Growth
- Regulation Of Autophagy
- Secretion
- Cell Killing
PTMs
- Phosphorylation
- Cleavage
- Ubiquitination
- Dephosphorylation
- Reduction
- Acetylation
Research Areas
- Apoptosis
- Autophagy
- Cancer
- Cell Biology
- Ubiquitin Proteasome Pathway
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ATG5
bosterbio.com/bosterbio-gene-info-cards/ATG5
References
PMID: 9563500 by Hammond E.M., et al. Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast.
PMID: 7796880 by Grand R.J.A., et al. A novel protein expressed in mammalian cells undergoing apoptosis.
8 Publications of Boster Autophagy protein 5 Antibodies & ELISA Kits
- PubMed ID: -, Gang Zhong,Huiping Long,Shiting Ma,Yao Shunhan,Jia Li,Jun Yao,miRNA-335-5p relie...
- PubMed ID: -, Shen W,Jia N,Miao J,Chen S,Zhou S,Meng P,Zhou X,Tang L,Zhou L:Penicilliumin B Pr...
- PubMed ID: 28848439, Antagomirs Targeting MiroRNA-134 Attenuates Epilepsy in Rats through Regulation ...
- PubMed ID: 28839236, Association between genetic polymorphisms in the autophagy-related 5 gene promot...
- PubMed ID: 27383629, Nrf2 signalling andautophagyare involved in diabetes mellitus-induced defects in...
- PubMed ID: 27163719, Proper autophagy is indispensable for angiogenesis during chick embryo developme...
- PubMed ID: 26459718, Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model ...
- PubMed ID: 29113167, Yang J, Sheng S, Yang Q, Li L, Qin S, Yu S, Zhang X. Oncol Lett. 2017 Nov;14(5):...
*More publications can be found for each product on its corresponding product page