Anti-FGF21 Biotinylated Antibody

FGF-21 antibody

Boster Bio Anti-FGF21 Biotinylated Antibody catalog # A00802-Biotin. Tested in ELISA applications. This antibody reacts with Human.

Product Info Summary

SKU: A00802-Biotin
Size: 50μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA

Product Name

Anti-FGF21 Biotinylated Antibody

View all FGF-21 Antibodies

SKU/Catalog Number

A00802-Biotin

Size

50μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FGF21 Biotinylated Antibody catalog # A00802-Biotin. Tested in ELISA applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FGF21 Biotinylated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00802-Biotin)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human FGF21 recombinant protein (Position: H29-S209).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00802-Biotin is reactive to FGF21 in Human

Applications

A00802-Biotin is guaranteed for ELISA Boster Guarantee

Background of FGF-21

FGF21 (Fibroblast growth factor 21) is a protein that in humans is encoded by the FGF21 gene, which is also a member of the fibroblast growth factor (FGF) family. The FGF21 gene is mapped on 19q13.33. Using RT-PCR, Fgf21 was expressed in several types of adipose tissue in mice, including subcutaneous and epididymal fat pads and brown adipose tissue. The level of Fgf21 expression in adipose tissue was comparable to that in liver. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Changes in Fgf21 expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels. In differentiated mouse brown adipocytes in culture, Fgf21 treatment increased the expression of thermogenic genes, caused higher total and uncoupled respiration, and enhanced glucose oxidation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA Detection (Matched Antibody Pair), 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For FGF21 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NSA1

Gene ID

26291

Gene Name

FGF21

Full Name

Fibroblast growth factor 21

Weight

22.3kDa

Superfamily

heparin-binding growth factors family

Alternative Names

FGF21; FGF-21; fibroblast growth factor 21 FGF21 fibroblast growth factor 21 fibroblast growth factor 21

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FGF21, check out the FGF21 Infographic

FGF21 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FGF21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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3 Customer Q&As for Anti-FGF21 Biotinylated Antibody

Question

We have seen staining in human right lobe of liver. Any tips? Is anti-FGF21 Biotinylated antibody supposed to stain right lobe of liver positively?

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

Based on literature right lobe of liver does express FGF21. Based on Uniprot.org, FGF21 is expressed in right lobe of liver, lung, among other tissues. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-03-15

Question

We were well pleased with the WB result of your anti-FGF21 Biotinylated antibody. However we have observed positive staining in right lobe of liver secreted. using this antibody. Is that expected? Could you tell me where is FGF21 supposed to be expressed?

Z. Wu

Verified customer

Asked: 2019-01-30

Answer

From literature, right lobe of liver does express FGF21. Generally FGF21 expresses in secreted. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-01-30

Question

We are currently using anti-FGF21 Biotinylated antibody A00802-Biotin for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?

A. Zhao

Verified customer

Asked: 2017-05-16

Answer

The anti-FGF21 Biotinylated antibody (A00802-Biotin) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-16

Size

Total: $315

SKU:A00802-Biotin

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A00802-Biotin
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