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Product Info Summary
| SKU: | EK1759 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Human Ki67 ELISA Kit PicoKine®
View all Ki67/MKI67 ELISA kits
SKU/Catalog Number
EK1759
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Ki67 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MKI67 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Ki67 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1759)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: N3120-I3256
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1759 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1759 is reactive to MKI67 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1759 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ki67/MKI67
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. This gene is mapped to 10q26.2. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1759-CAP | Anti-Human MKI67 Pre-coated 96-well strip microplate | 1 |
| EK1759-ST | Human MKI67 Standard | 2 vials, 1 ng/tube |
| EK1759-DA | Human MKI67 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.026 | 0.071 | 0.124 | 0.193 | 0.375 | 0.698 | 1.263 | 2.076 |
Data Example Images
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Human Ki67 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Ki67/MKI67, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 7.8 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Ki67 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 15 | 54 | 222 | 16 | 60 | 234 |
| Standard deviation | 0.9 | 3.13 | 10.4 | 0.94 | 4.2 | 18.25 |
| CV (%) | 6.3% | 5.8% | 4.7% | 5.9% | 7.0% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Ki67/MKI67 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 15 | 17 | 17 | 15 | 16 | 1.00 | 6.2% |
| Sample 2 | 54 | 64 | 64 | 54 | 59 | 5.00 | 8.4% |
| Sample 3 | 222 | 213 | 228 | 245 | 227 | 11.68 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For MKI67 (Source: Uniprot.Org, NCBI)
Gene Name
MKI67
Full Name
Proliferation marker protein Ki-67
Weight
358.694kDa
Alternative Names
antigen identified by monoclonal Ki-67; Antigen Ki67; antigen KI-67; antiKi67; Ki67 ihc; Ki-67 ihc; Ki67 mouse; Ki-67 mouse; Ki67 western blot; Ki-67 western blot; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation Marker Protein Ki-67; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126 MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67|antigen Ki67|antigen identified by monoclonal antibody Ki-67|proliferation-related Ki-67 antigen|protein phosphatase 1, regulatory subunit 105
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MKI67, check out the MKI67 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MKI67: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Ki67 ELISA Kit PicoKine® (EK1759)
Hello CJ!
EK1759 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Efficacy of EP chemotherapy followed by IP chemotherapy combined with radiotherapy in the treatment of extensive-stage small-cell lung cancer. Shiyang Kang,Chaopeng Ou,Ruifeng Xue,Weian Zeng,Jingxiu Huang,Yingjun Zhang,Dongtai Chen,Jielan Lai
Species: Human
EK1759 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human Ki67 ELISA Kit PicoKine®
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-11-11
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-11-11
Question
Q: can you recommend the dilution ratio of serum samples for detection of Ki67 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Ki67 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-02-10
Question
Q: if the enzyme conjugated Ki67 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Ki67 antigen?
Verified Customer
Verified customer
Asked: 2019-08-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-08-25
Question
Q: how do I analyze ELISA data? I have performed Ki67 level in plasma.
S. Puri
Verified customer
Asked: 2018-12-18
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-12-18
Question
Q: Can Ki67 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-07-14
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-07-14
Question
Q: What is the optimal O.D. value for Ki67 ELISA kit? I performed your Ki67 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Ki67 even though the O.D. values are not very high?
B. Clark
Verified customer
Asked: 2017-08-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Ki67 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of Ki67 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2017-08-05
Question
Q: is it okay to use citrate plasma as samples in Human Ki67 Picokine® ELISA Kit (Catalog # EK1759)?
Verified Customer
Verified customer
Asked: 2017-01-13
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Ki67 causing degradation of its protein structure. Ki67 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Ki67 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-01-13
