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Product Info Summary
| SKU: | EK0950 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human MMP-12 ELISA Kit PicoKine®
SKU/Catalog Number
EK0950
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MMP-12 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MMP-12 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0950)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L17-C470
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0950 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0950 is reactive to MMP12 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0950 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-12
Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0950-CAP | Anti-Human MMP12 Pre-coated 96-well strip microplate | 1 |
| EK0950-ST | Human MMP12 Standard | 2 vials, 10 ng/tube |
| EK0950-DA | Human MMP12 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.049 | 0.136 | 0.203 | 0.306 | 0.564 | 0.965 | 1.571 | 2.080 |
Data Example Images
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Human MMP-12 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-12, Dilution ratio of 1:1, concentration in serum and plasma is around 500 pg/ml..
Some articles we found to cite concentrations of MMP-12 in samples: 16308335 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-12 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 201 | 499 | 1029 | 197 | 507 | 1084 |
| Standard deviation | 12.86 | 20.45 | 59.68 | 13.39 | 30.42 | 62.87 |
| CV (%) | 6.4% | 4.1% | 5.8% | 6.8% | 6% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-12 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 201 | 172 | 181 | 197 | 187 | 11.77 | 6.2% |
| Sample 2 | 499 | 501 | 511 | 504 | 503 | 4.54 | 0.9% |
| Sample 3 | 1029 | 985 | 961 | 957 | 983 | 28.63 | 2.9% |
Protein Target Info & Infographic
Gene/Protein Information For MMP12 (Source: Uniprot.Org, NCBI)
Gene Name
MMP12
Full Name
Macrophage metalloelastase
Weight
54.002kDa
Superfamily
peptidase M10A family
Alternative Names
EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12 MMP12 HME, ME, MME, MMP-12 matrix metallopeptidase 12 macrophage metalloelastase|matrix metallopeptidase 12 (macrophage elastase)|matrix metalloproteinase 12 (macrophage elastase)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MMP12, check out the MMP12 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MMP-12 ELISA Kit PicoKine® (EK0950)
Hello CJ!
EK0950 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Elastin‑derived peptides are involved in the processes of human temporomandibular disorder by inducing inflammatory responses in synovial cells
Acute Exacerbations of COPD Are Associated With Increased Expression of Heparan Sulfate and Chondroitin Sulfate in BAL
Expressions of MMP-12, TIMP-4, and Neutrophil Elastase in PBMCs and Exhaled Breath Condensate in Patients with COPD and Their Relationships with Disease Severity and Acute Exacerbations
Concomitant elevations of MMP‐9, NGAL, proMMP‐9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease
Impact of cathepsin D activity and C224T polymorphism (rs17571) on chronic obstructive pulmonary disease: correlations with oxidative and inflammatory markers
MMP‐3 (–1171 5A/6A; Lys45Glu) variants affect serum levels of matrix metalloproteinase (MMP)‐3 and correlate with severity of COPD: A study of MMP‐3, MMP‐7 and MMP‐12 in a Tunisian population
Bchir S,Boumiza S,Ben Nasr H,Garrouch A,Kallel I,Tabka Z,Chahed K.Impact of cathepsin D activity and C224T polymorphism (rs17571) on chronic obstructive pulmonary disease: correlations with oxidative and inflammatory markers.Clin Exp Med.2021 Feb 21.doi:1
Species: Human
EK0950 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Li J,Xie Y,Zhao P,Qin Y,Oliver BG,Tian Y,Li S,Wang M,Liu X. A chinese herbal formula ameliorates COPD by inhibiting the inflammatory response via downregulation of p65, JNK, and p38. Phytomedicine. 2021 Jan 20;83:153475.doi:10.1016/j.phymed.2021.153475.Ep
Species: Human,Rat
EK0950 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA
Elastin-derived peptides are involved in the processes of human temporomandibular disorder by inducing inflammatory responses in synovial cells
Concomitant elevations of MMP?9, NGAL, proMMP?9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
9 Customer Q&As for Human MMP-12 ELISA Kit PicoKine®
Question
I'm wondering if you have the sequences for the immunogens used to raise the antibodies in Human MMP-12 ELISA Kit EK095
Verified customer
Asked: 2022-09-06
Answer
The immunogen sequence of both the capture and detection antibodies in kit EK0950 was L17-C470.
Boster Scientific Support
Answered: 2022-09-06
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-04-24
Question
Are the standards in EK0950 natural material or recombinant? If it's recombinant, what kind of cell or living material is used for expression?
Verified customer
Asked: 2020-03-13
Answer
The standard of the Human MMP-12 ELISA Kit PicoKine EK0950 is recombinant and the expression system is NSO.
Boster Scientific Support
Answered: 2020-03-13
Question
Was a virus vector used in the recombination and/or expression process of EK0950? What kind?
Verified customer
Asked: 2020-03-13
Answer
There wasn't any virus vector used in the recombination and/or expression process of the Human MMP-12 ELISA Kit PicoKine EK0950.
Boster Scientific Support
Answered: 2020-03-13
Question
Do the standards in EK0950 include additive agent such as antiseptic or preservative? What is the composition of the additive agent?
Verified customer
Asked: 2020-03-13
Answer
The standard of the Human MMP-12 ELISA Kit PicoKine EK0950 contains 0.02% sodium azide. The buffer prior to lyophilization contains 0.02% sodium azide, 2% BSA, 0.2% Tris and 1% NaCl.
Boster Scientific Support
Answered: 2020-03-13
Question
What is the material name and concentration of Stop Solution for EK0950?
Verified customer
Asked: 2020-03-13
Answer
The Stop Solution for the Human MMP-12 ELISA Kit PicoKine EK0950 is 2% Sulphuric acid.
Boster Scientific Support
Answered: 2020-03-13
Question
Does EK0950 kit include hazardous substance such as azide, thimerosal, 2-ME, etc?
Verified customer
Asked: 2020-03-13
Answer
The Human MMP-12 ELISA Kit PicoKine EK0950 kit contains sodium azide (0.02%), sulphuric acid (2%) and proclin(0.2%).
Boster Scientific Support
Answered: 2020-03-13
Question
Q: What is the optimal O.D. value for MMP-12 ELISA kit? I used your MMP-12 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain MMP-12 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MMP-12 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MMP-12 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-01-07
Question
Q: can you suggest the dilution ratio of serum samples for detection of MMP-12 in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MMP-12 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-07
