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Product Info Summary
| SKU: | EK0544 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine®
View all VEGFR2/KDR/Flk-1 ELISA kits
SKU/Catalog Number
EK0544
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human KDR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0544)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A20-E764
Sensitivity
<4 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0544 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0544 is reactive to KDR in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0544 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFR2/KDR/Flk-1
Vascular endothelial growth factor receptor 2 (VEGFR-2) also known as Kinase insert domain receptor (KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0544-CAP | Anti-Human KDR Pre-coated 96-well strip microplate | 1 |
| EK0544-ST | Human KDR Standard | 2 vials, 10 ng/tube |
| EK0544-DA | Human KDR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.065 | 0.115 | 0.165 | 0.265 | 0.446 | 0.814 | 1.517 | 2.062 |
Data Example Images
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Human VEGFR2/KDR PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VEGFR2/KDR/Flk-1, Dilution ratio of 1:10, concentration in serum and plasma is 5136-17401 pg/ml..
Some articles we found to cite concentrations of VEGFR2/KDR/Flk-1 in samples: 19551437 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 177 | 400 | 1762 | 167 | 410 | 1810 |
| Standard deviation | 10.79 | 26 | 137.43 | 11.52 | 29.52 | 161.09 |
| CV (%) | 6.1% | 6.5% | 7.8% | 6.9% | 7.2% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of VEGFR2/KDR/Flk-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 177 | 180 | 169 | 166 | 173 | 5.7 | 3.2% |
| Sample 2 | 400 | 400 | 444 | 444 | 422 | 22 | 5.2% |
| Sample 3 | 1762 | 1652 | 1818 | 1564 | 1699 | 98.18 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For KDR (Source: Uniprot.Org, NCBI)
Gene Name
KDR
Full Name
Vascular endothelial growth factor receptor 2
Weight
151.527kDa
Superfamily
protein kinase superfamily
Alternative Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2 KDR CD309, FLK1, VEGFR, VEGFR2 kinase insert domain receptor vascular endothelial growth factor receptor 2|fetal liver kinase-1|kinase insert domain receptor (a type III receptor tyrosine kinase)|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on KDR, check out the KDR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KDR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine® (EK0544)
Hello CJ!
EK0544 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene
eIF3i activity is critical for endothelial cells in tumor induced angiogenesis through regulating VEGFR and ERK translation
Vascular endothelial growth factor gene polymorphisms in patients with rosacea: A case-control study
Synthesis, anticancer effect and molecular modeling of new thiazolylpyrazolyl coumarin derivatives targeting VEGFR-2 kinase and inducing cell cycle arrest and apoptosis
Role of the HIF%u20111 signaling pathway in chronic obstructive pulmonary disease
eIF3i activity is critical for endothelial cells in tumor induced angiogenesis through regulating VEGFR and ERK translation
Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene
Wang L, Liu X, Wang H, Wang S. Oncol Lett. 2013 Jul;6(1):175-180. Epub 2013 May 15. Correlation Of The Expression Of Vascular Endothelial Growth Factor And Its Receptors With Microvessel Density In Ovarian Cancer.
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Human VEGFR2 Picokine® ELISA Kit (Catalog # EK0544)?
C. Yang
Verified customer
Asked: 2019-11-03
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of VEGFR2 causing disruption of its protein structure. VEGFR2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the VEGFR2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-11-03
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
P. Jackson
Verified customer
Asked: 2019-10-03
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-10-03
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Bhasin
Verified customer
Asked: 2019-03-04
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-03-04
Question
Q: how to proceed with the analysis of ELISA data? I have obtained VEGFR2 level in plasma.
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-06-22
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGFR2 using the EK0544 Human VEGFR2 Picokine® ELISA Kit?
M. Williams
Verified customer
Asked: 2018-03-04
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-03-04
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-05-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-05-12
