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Product Info Summary
| SKU: | FEK0527 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Mouse TNF Alpha/Tumor necrosis factor PicoKine™ One-Step Quick ELISA Kit
SKU/Catalog Number
FEK0527
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TNF Alpha/Tumor necrosis factor PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNF Alpha/Tumor necrosis factor PicoKine™ One-Step Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0527)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: L80-L235
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml (cell culture supernatants), 7.8 pg/ml - 500 pg/ml (mouse serum, plasma)
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0527 is reactive to TNF in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK0527 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TNF-alpha
Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte- macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
Kit Components
| Catalog number | Description | Quantity |
|---|---|---|
| FEK0527-CAP | 96-well plate precoated with anti-Mouse TNF-alpha antibody | 1 |
| EK0527-ST | lyophilized recombinant Mouse TNF-alpha standard | 10 ng/tube |
| FEK0527-DA | Biotinylated anti-Mouse TNF-alpha detection antibody (50x) | 100 ul |
| - | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
| AR1106-1 | Sample diluent | 30 ml |
| AR1106-2 | Antibody diluent | 12 ml |
| - | Avidin-Biotin-Peroxidase Diluent | 12 ml |
| AR1106-5 | Wash Buffer (25x) | 20 ml |
| AR1104 | Color Developing Reagent (TMB) | 10 ml |
| AR1105 | Stop Solution | 10 ml |
| PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.160 | 0.232 | 0.311 | 0.406 | 0.654 | 1.065 | 1.673 | 2.430 |
Data Example Images
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Mouse TNF alpha PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 20 | 55 | 314 | 18 | 63 | 350 |
| Standard deviation | 1.04 | 3.74 | 23.55 | 1.06 | 4.59 | 28.7 |
| CV (%) | 5.2% | 6.8% | 7.5% | 5.9% | 7.3% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TNF-alpha in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 20 | 22 | 18 | 19 | 19 | 0.89 | 4.7% |
| Sample 2 | 55 | 58 | 64 | 57 | 58 | 3.65 | 6.3% |
| Sample 3 | 314 | 297 | 303 | 287 | 300 | 8.1 | 2.7% |
Protein Target Info & Infographic
Gene/Protein Information For TNF (Source: Uniprot.Org, NCBI)
Uniprot ID
P01375
Gene ID
7124
Gene Name
TNF
Full Name
Tumor necrosis factor
Weight
25.644kDa
Superfamily
Tumor necrosis factor family
Alternative Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNF, check out the TNF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TNF Alpha/Tumor necrosis factor PicoKine™ One-Step Quick ELISA Kit (FEK0527)
Hello CJ!
FEK0527 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu R, Tao A, Zhang S, Zhang M. Acta Biochim Biophys Sin (Shanghai). 2013 Sep;45(9):726-33. Doi: 10.1093/Abbs/Gmt065. Epub 2013 Jun 19. Neutralization Of Interleukin-17 Attenuates High Fat Diet-Induced Non-Alcoholic Fatty Liver Disease In Mice.
Zhang H, Xia X, Han F, Jiang Q, Rong Y, Song D, Wang Y. Mol Pharm. 2015 May 4;12(5):1648-61. Doi: 10.1021/Acs.Molpharmaceut.5B00069. Epub 2015 Apr 2. Cathelicidin-Bf, A Novel Antimicrobial Peptide From Bungarus Fasciatus, Attenuates Disease In A D...
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