Product Info Summary
SKU: | PROTP01237 |
---|---|
Size: | 10ug, 50ug, 1mg |
Origin Species: | Rat |
Source: | Escherichia coli |
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Product info
Product Name
Prolactin Rat Recombinant Protein
View all Prolactin recombinant proteins
SKU/Catalog Number
PROTP01237
Size
10ug, 50ug, 1mg
Description
Prolactin Rat Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 198 amino acids and having a molecular mass of 22.6 kDa. The Prolactin is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prolactin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Cite This Product
Prolactin Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP01237)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 10mM sodium Phosphate buffer pH=8 and 50mM Nacl.
Purity
Greater than 95.0% as determined by (a) Analysis by SEC-HPLC and (b) Analysis by SDS-PAGE.
Predicted MW
25.876kDa
Reconstitution
It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Val-Cys.
Biological Activity
The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be less than 0.065 ng/ml corresponding to a specific activity of 15,400,000 Units/mg.
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Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For PRL (Source: Uniprot.org, NCBI)
Gene Name
PRL
Full Name
Prolactin
Weight
25.876kDa
Superfamily
somatotropin/prolactin family
Alternative Names
PRL; Prolactin PRL GHA1 prolactin prolactin|decidual prolactin|growth hormone A1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PRL, check out the PRL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Prolactin Rat Recombinant Protein (PROTP01237)
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No publications found for PROTP01237
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Customer Q&As
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1 Customer Q&As for Prolactin Rat Recombinant Protein
Question
How does PROTP01237 differ from PROTP01237-1?
Verified customer
Asked: 2021-02-23
Answer
For the Prolactin Rat Recombinant Protein (PROTP01237), the protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbance value of 0.93 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by a DNAman computer analysis program of protein sequences.
Boster Scientific Support
Answered: 2021-02-24