Prolactin Rat Recombinant Protein

Prolactin protein, Rat

Prolactin Rat Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 198 amino acids and having a molecular mass of 22.6 kDa. The Prolactin is purified by proprietary chromatographic techniques.

Product Info Summary

SKU: PROTP01237
Size: 10ug, 50ug, 1mg
Origin Species: Rat
Source: Escherichia coli

Product Name

Prolactin Rat Recombinant Protein

View all Prolactin recombinant proteins

SKU/Catalog Number

PROTP01237

Size

10ug, 50ug, 1mg

Description

Prolactin Rat Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 198 amino acids and having a molecular mass of 22.6 kDa. The Prolactin is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prolactin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

Prolactin Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP01237)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution with 10mM sodium Phosphate buffer pH=8 and 50mM Nacl.

Purity

Greater than 95.0% as determined by (a) Analysis by SEC-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

25.876kDa

Reconstitution

It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Amino Acid Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Val-Cys.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be less than 0.065 ng/ml corresponding to a specific activity of 15,400,000 Units/mg.

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Reconstitution

It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For PRL (Source: Uniprot.org, NCBI)

Gene Name

PRL

Full Name

Prolactin

Weight

25.876kDa

Superfamily

somatotropin/prolactin family

Alternative Names

PRL; Prolactin PRL GHA1 prolactin prolactin|decidual prolactin|growth hormone A1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on PRL, check out the PRL Infographic

PRL infographic

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In this infographic, you will see the following information for PRL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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1 Customer Q&As for Prolactin Rat Recombinant Protein

Question

How does PROTP01237 differ from PROTP01237-1?

Verified customer

Asked: 2021-02-23

Answer

For the Prolactin Rat Recombinant Protein (PROTP01237), the protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbance value of 0.93 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by a DNAman computer analysis program of protein sequences.

Boster Scientific Support

Answered: 2021-02-24

Size

Total: $250

SKU:PROTP01237

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Lead time for this item is typically 10-14 days

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