Anti-Phospho-5-Lipoxygenase (Ser523) ALOX5 Antibody

5-Lipoxygenase antibody

Boster Bio Anti-Phospho-5-Lipoxygenase (Ser523) ALOX5 Antibody (Catalog # P00847). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P00847
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Phospho-5-Lipoxygenase (Ser523) ALOX5 Antibody

View all 5-Lipoxygenase Antibodies

SKU/Catalog Number

P00847

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-5-Lipoxygenase (Ser523) ALOX5 Antibody (Catalog # P00847). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-5-Lipoxygenase (Ser523) ALOX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00847)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser523 of human 5-lipoxygenase (5-LO), conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00847 is reactive to ALOX5 in Human, Mouse, Rat

Applications

P00847 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

77.983kDa

Background of 5-Lipoxygenase

The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For ALOX5 (Source: Uniprot.org, NCBI)

Gene Name

ALOX5

Full Name

Arachidonate 5-lipoxygenase

Weight

77.983kDa

Superfamily

lipoxygenase family

Alternative Names

5Lipoxygenase; 5-Lipoxygenase; 5-LO; 5-LOX; 5LPG; ALOX5; arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; EC 1.13.11; EC 1.13.11.34; leukotriene A4 synthase; LOG5; LOX5A; MGC163204 ALOX5 5-LO, 5-LOX, 5LPG, LOG5 arachidonate 5-lipoxygenase polyunsaturated fatty acid 5-lipoxygenase|LOX-5|arachidonic acid 5-lipoxygenase|leukotriene A4 synthase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALOX5, check out the ALOX5 Infographic

ALOX5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALOX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Size

Total: $465

SKU:P00847

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Lead time for this item is typically 6-8 days

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P00847
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$465.00

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