Anti-67kDa Laminin Receptor RPSA Rabbit Monoclonal Antibody

Product Name

Anti-67kDa Laminin Receptor RPSA Rabbit Monoclonal Antibody

View all RPSA Antibodies

SKU/Catalog Number

M01691

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-67kDa Laminin Receptor RPSA Rabbit Monoclonal Antibody catalog # M01691. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-67kDa Laminin Receptor RPSA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01691)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOAO-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human 67kDa Laminin Receptor

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01691 is reactive to RPSA in Human, Mouse, Rat

Applications

M01691 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

v

Calculated molecular weight

32.854kDa

Background of RPSA

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of 67kDa Laminin Receptor using anti-67kDa Laminin Receptor antibody (M01691).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human COLO320 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-67kDa Laminin Receptor antigen affinity purified monoclonal antibody (Catalog # M01691) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 67kDa Laminin Receptor at approximately 40 kDa. The expected band size for 67kDa Laminin Receptor is at 33 kDa.

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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.

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Western blot analysis of 67kDa Laminin Receptor expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.

Gene/Protein Information For RPSA (Source: Uniprot.org, NCBI)

Alternative Names

37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRPNEM/1CHD4; 67 kDa laminin receptor; 67kD, ribosomal protein SA; 67LR; Colon carcinoma laminin-binding protein; LAMBR37 kDa laminin receptor; laminin binding protein; laminin receptor 1 (67kD, ribosomal protein SA); Laminin receptor 1; Laminin-binding protein precursor p40; LAMR 1 ; lamR; LAMR140S ribosomal protein SA; LBP; LBP/p40; LRP; LRP/LR; Multidrug resistance-associated protein MGr1-Ag; p40; ribosomal protein SA RPSA 37LRP, 67LR, ICAS, LAMBR, LAMR1, LBP, LBP/p40, LRP, LRP/LR, NEM/1CHD4, SA, lamR, p40 ribosomal protein SA 40S ribosomal protein SA|37 kDa laminin receptor|37/67 kDa laminin receptor|67 kDa laminin receptor|colon carcinoma laminin-binding protein|laminin receptor 1 (67kD, ribosomal protein SA)|laminin-binding protein precursor p40|multidrug resistance-associated protein MGr1-Ag|small ribosomal subunit protein uS2

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