Product Info Summary
SKU: | P00024-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Drosophila |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-AKT (Thr342) AKT1 Antibody
SKU/Catalog Number
P00024-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-AKT (Thr342) AKT1 Antibody (Catalog # P00024-1). Tested in WB applications. This antibody reacts with Drosophila.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-AKT (Thr342) AKT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00024-1)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 of Drosophila AKT, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Drosophila.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00024-1 is reactive to AKT1 in Drosophila
Applications
P00024-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55.686kDa
Background of Akt1
The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (α, β, and γ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktα occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
Validation Images & Assay Conditions
Click image to see more details
Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 minutes).
Protein Target Info & Infographic
Gene/Protein Information For AKT1 (Source: Uniprot.org, NCBI)
Gene Name
AKT1
Full Name
RAC-alpha serine/threonine-protein kinase
Weight
55.686kDa
Superfamily
protein kinase superfamily
Alternative Names
AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT1, check out the AKT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-AKT (Thr342) AKT1 Antibody (P00024-1)
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No publications found for P00024-1
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Phospho-AKT (Thr342) AKT1 Antibody
Question
We are currently using anti-Phospho-AKT (Thr342) antibody P00024-1 for drosophila tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says drosophila. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The anti-Phospho-AKT (Thr342) antibody (P00024-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-28
Question
We have observed staining in drosophila muscle ovary. Any tips? Is anti-Phospho-AKT (Thr342) antibody supposed to stain muscle ovary positively?
B. Zhao
Verified customer
Asked: 2017-10-23
Answer
From literature muscle ovary does express AKT1. From Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-10-23
Question
Our lab were content with the WB result of your anti-Phospho-AKT (Thr342) antibody. However we have been able to see positive staining in muscle ovary cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?
C. Jones
Verified customer
Asked: 2016-03-07
Answer
From what I have seen in literature, muscle ovary does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-03-07