Anti-Apaf1 Antibody

Product Name

Anti-Apaf1 Antibody

View all APAF-1 Antibodies

SKU/Catalog Number

A00889

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Apaf1 Antibody (Catalog # A00889). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Apaf1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Apaf1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00889)

Host

Rabbit

Contents

Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse. The immunogen is located within the first 50 amino acids of Apaf1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

IKK gamma has no cross response to IKK alpha or IKK beta.

Reactive Species

A00889 is reactive to APAF1 in Human, Mouse, Rat

Applications

A00889 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

141.84kDa

Background of APAF-1

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 μg/ml.

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Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/mL.

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Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 μg/mL.

Gene/Protein Information For APAF1 (Source: Uniprot.org, NCBI)

Alternative Names

APAF1; APAF-1; APAF-1apoptotic protease activating factor 1; apoptotic peptidase activating factor 1; apoptotic peptidase activating factor; apoptotic protease activating factor; apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413 APAF1 APAF-1, CED4 apoptotic peptidase activating factor 1 apoptotic protease-activating factor 1

For more info on APAF1, check out the APAF1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for APAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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