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Product Info Summary
| SKU: | A01263-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-beta-Actin ACTB Antibody
View all beta-Actin Antibodies
SKU/Catalog Number
A01263-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-beta-Actin ACTB Antibody (Catalog # A01263-2). Tested in ELISA, WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-beta-Actin ACTB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01263-2)
Host
Rabbit
Contents
beta-Actin Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
b-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human b-actin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.
Reactive Species
A01263-2 is reactive to ACTB in Human, Mouse, Rat
Applications
A01263-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
41.737kDa
Background of beta-Actin
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of beta-Actin in A431
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Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 μg/mL.
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Immunofluorescence of beta-Actin in Hela cells with beta-Actin antibody at 2 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41.737kDa
Superfamily
actin family
Alternative Names
ACTB; actin, beta; actin, cytoplasmic 1; beta actin; B-Actin; beta actin (ac-15); Beta Actin; beta cytoskeletal actin; beta-actin ac 15; beta-actin ac15; beta-actin ac-15; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1 ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta-Actin ACTB Antibody (A01263-2)
Hello CJ!
A01263-2 has been cited in 74 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang H,Lu Z,Huo C,Chen Y,Cao H,Xie P,Zhou H,Liu D,Liu J,Yu L.Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21.Front Pharmacol.2019 Nov 14;10:1332.doi:10.3389/fphar.2019.01332.PMID:31803051;PMCID:PMC6868520.
Species: Mouse
A01263-2 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
Guo J,Tu J,Hu Y,Song G,Yin Z.Cathepsin G cleaves and activates IL-36γ and promotes the inflammation of psoriasis.Drug Des Devel Ther.2019 Feb 8;13:581-588.doi:10.2147/DDDT.S194765.PMID:30804664;PMCID:PMC6372007.
Species: Human,Mouse
Li R,Gong Z,Yu Y,Niu R,Bian S,Sun Z.Alleviative Effects of Exercise on Bone Remodeling in Fluorosis Mice.Biol Trace Elem Res.2021 May 3.doi:10.1007/s12011-021-02741-y.Epub ahead of print.PMID:33939130.
Species: Mouse
A01263-2 usage in article: APP:WB, SAMPLE:FEMUR TISSUE, DILUTION: 1:4000
Xianpeng Li,Huaixi Yu,Feng Xu,Yifeng Wu,Jifang Sheng,"Differentially Expressed Long Noncoding RNAs Involved in FUBP1 Promoting Hepatocellular Carcinoma Cells Proliferation",BioMed Research International,vol.2021,Article ID 6664519,9 pages,2021.https://doi.org/10.1155/2021/6664519
Species: Human
A01263-2 usage in article: APP:WB, SAMPLE:HCC CELL, DILUTION:1:2000
Chen, G., Liu, Y., Xu, Y., Zhang, M., Guo, S., & Zhang, G. (2021). Isoimperatorin exerts anti-inflammatory activity by targeting the LPS-TLR4/MD-2-NF-κB pathway. European Journal of Inflammation. https://doi.org/10.1177/20587392211000573
Species: Mouse
A01263-2 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
Peng L,Liang Y,Zhong X,Liang Z,Tian Y,Li S,Liang J,Wang R,Zhong Y,Shi Y,Zhang X.Aptamer-Conjugated Gold Nanoparticles Targeting Epidermal Growth Factor Receptor Variant III for the Treatment of Glioblastoma.Int J Nanomedicine.2020 Feb 28;15:1363-1372.doi:
Species: Human,Mouse
A01263-2 usage in article: APP:WB, SAMPLE:U87-EGFRVIII CELL, DILUTION:NA
Gu W,Shi Z,Song G,Zhang H.MicroRNA-199-3p up-regulation enhances chondrocyte proliferation and inhibits apoptosis in knee osteoarthritis via DNMT3A repression.Inflamm Res.2021 Jan 12.doi:10.1007/s00011-020-01430-1.Epub ahead of print.PMID:33433641.
Species: Rat
A01263-2 usage in article: APP:WB, SAMPLE:CHONDROCYTE, DILUTION:1:2500
Wang S,Fan X,Zhu J,Xu D,Li R,Chen R,Hu J,Shen Y,Hao J,Wang K,Jiang X,Wang Y,Jiang Y,Li J,Zhang J.The differentiation of colorectal cancer is closely relevant to m6A modification.Biochem Biophys Res Commun.2021 Feb 8;546:65-73.doi:10.1016/j.bbrc.2021.02.00
Species: Human
A01263-2 usage in article: APP:WB, SAMPLE:CACO2 CELL AND HCT116 CELL, DILUTION:1:1000
Wang S,Fan X,Zhu J,Xu D,Li R,Chen R,Hu J,Shen Y,Hao J,Wang K,Jiang X,Wang Y,Jiang Y,Li J,Zhang J.The differentiation of colorectal cancer is closely relevant to m6A modification.Biochem Biophys Res Commun.2021 Feb 8;546:65-73.doi:10.1016/j.bbrc.2021.02.00
Species: Human
A01263-2 usage in article: APP:WB, SAMPLE:CACO2 CELL AND HCT116 CELL, DILUTION:1:1000
Meng J,Su R,Wang L,Yuan B,Li L.2021.Inhibitory effect and mechanism of action (MOA) of hirsutine on the proliferation of T-cell leukemia Jurkat clone E6-1 cells.PeerJ 9:e10692 https://doi.org/10.7717/peerj.10692
Species: Human
A01263-2 usage in article: APP:WB, SAMPLE:JURKAT CLONE E6 CELL, DILUTION:1:1000
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