Product Info Summary
SKU: | A01469-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BNIP3 Antibody
SKU/Catalog Number
A01469-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BNIP3 Antibody catalog # A01469-2. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BNIP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01469-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ACVL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01469-2 is reactive to BNIP3 in Human, Mouse, Rat
Applications
A01469-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
27.832kDa
Background of BNIP3
Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
ten Dijke P., Oncogene 8:2879-2887(1993).
Attisano L., Cell 75:671-680(1993).
Berg J.N., Am. J. Hum. Genet. 61:60-67(1997).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of BNIP3 using Anti-BNIP3 Antibody (A01469-2).
ICC staining BNIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-BNIP3 Antibody A01469-2
Western blot analysis of BNIP3 on different cells lysates using anti-BNIP3 antibody at 1/500 dilution. Positive control:Line 1: Human skeletal muscle
Line 2: Mouse kidney
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For BNIP3 (Source: Uniprot.org, NCBI)
Gene Name
BNIP3
Full Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Weight
27.832kDa
Superfamily
NIP3 family
Alternative Names
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3; BCL2/adenovirus E1B 19kD-interacting protein 3; BNIP3; Nip3 BNIP3 NIP3 BCL2 interacting protein 3 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3|BCL2/adenovirus E1B 19kDa interacting protein 3|BCL2/adenovirus E1B interacting protein 3|nineteen kD interacting protein-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BNIP3, check out the BNIP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BNIP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BNIP3 Antibody (A01469-2)
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No publications found for A01469-2
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