Product Info Summary
SKU: | A06435 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CD307 FCRL5 Antibody
View all FCRL5/FcRH5/IRTA2 Antibodies
SKU/Catalog Number
A06435
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD307 FCRL5 Antibody catalog # A06435. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD307 FCRL5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06435)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
6C12
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human CD307.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06435 is reactive to FCRL5 in Human
Applications
A06435 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
106.437kDa
Background of FCRL5/FcRH5/IRTA2
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Microtubules are polymers of tubulin, which exist as heterodimers of alpha-tubulin and beta-tubulin. NEDD1 (neural precursor expressed, developmentally down-regulated protein1; also called GCP-WD) is a centrosomal protein that in mammals associates with the gamma-tubulin ring complex. Gamma-TuRC is critical for initiation, or nucleation, of the microtubule assembly. In association with this complex, NEDD1 plays an important role in targeting the gamma-TuRC complex to the site of microtubule nucleation and to the mitotic spindle. These events are essential for proper bipolar spindle formation and mitotic progression. Given the casual link between improper spindle function and tumorigenesis, characterization of Nedd1 function will be important to better understand various mechanisms underlying mitotic regulation, chromosome segregation, and cancer development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC-p 1:100-300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
Click image to see more details
Western blot analysis of HepG2 cells using CD307 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
Click image to see more details
Western blot analysis of lysate from HepG2 cells, using FCRL5 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For FCRL5 (Source: Uniprot.org, NCBI)
Gene Name
FCRL5
Full Name
Fc receptor-like protein 5
Weight
106.437kDa
Alternative Names
BXMAS1; BXMAS1MGC119592; CD307; CD307e antigen; CD307e; DKFZp667E2019; DKFZp667F216; Fc receptor homolog 5; Fc receptor-like 5; Fc receptor-like protein 5; FcRH5; FCRH5MGC119593; FCRL5; FcR-like protein 5; FLJ00333; FLJ00397; IFGP5; Immune receptor translocation-associated protein 2; immunoglobulin superfamily receptor translocation associated 2 (IRTA2); IRTA2; IRTA2MGC119590; PRO820 FCRL5 BXMAS1, CD307, CD307e, FCRH5, IRTA2, PRO820 Fc receptor like 5 Fc receptor-like protein 5|fc receptor homolog 5|fcR-like protein 5|immune receptor translocation-associated protein 2|immunoglobulin superfamily receptor translocation associated 2 (IRTA2)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCRL5, check out the FCRL5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCRL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD307 FCRL5 Antibody (A06435)
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No publications found for A06435
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CD307 FCRL5 Antibody
Question
Is this A06435 anti-CD307 antibody reactive to the isotypes of FCRL5?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The immunogen of A06435 anti-CD307 antibody is Synthesized peptide derived from the Internal region of human CD307. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-17
Question
I was wanting to use your anti-CD307 antibody for WB for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
As indicated on the product datasheet, A06435 anti-CD307 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
Does anti-CD307 antibody A06435 work for WB with spleen?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
According to the expression profile of spleen, FCRL5 is highly expressed in spleen. So, it is likely that anti-CD307 antibody A06435 will work for WB with spleen.
Boster Scientific Support
Answered: 2019-03-21
Question
We are currently using anti-CD307 antibody A06435 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
The anti-CD307 antibody (A06435) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-07
Question
I see that the anti-CD307 antibody A06435 works with WB, what is the protocol used to produce the result images on the product page?
S. Jones
Verified customer
Asked: 2016-07-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-07-13