Product Info Summary
SKU: | A00672-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cytochrome P450 2E1 CYP2E1 Antibody
View all Cytochrome P450 2E1 Antibodies
SKU/Catalog Number
A00672-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 2E1 CYP2E1 Antibody catalog # A00672-2. Tested in WB,ICC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 2E1 CYP2E1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00672-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human p68 RNA Helicase around the phosphorylation site of Y593.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00672-2 is reactive to CYP2E1 in Human, Mouse, Rat
Applications
A00672-2 is guaranteed for ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
56.849kDa
Background of Cytochrome P450 2E1
Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements. Anti-Ppar Antibody is useful for research interested in transcription and metabolic pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
ICC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Cytochrome P450 2E1 CYP2E1 Antibody A00672-2
Western blot (WB) analysis of CYP2E1 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:H9C2 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CYP2E1 (Source: Uniprot.org, NCBI)
Gene Name
CYP2E1
Full Name
Cytochrome P450 2E1
Weight
56.849kDa
Superfamily
cytochrome P450 family
Alternative Names
4-nitrophenol 2-hydroxylase; CPE1; CYP2Ecytochrome P450 2E1; CYPIIE1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; Cytochrome P450-J; EC 1.14.13.-; EC 1.14.13.n7; EC 1.14.14.1; flavoprotein-linked monooxygenase; microsomal monooxygenase; P450C2E; P450-J; xenobiotic monooxygenase CYP2E1 CPE1, CYP2E, P450-J, P450C2E cytochrome P450 family 2 subfamily E member 1 cytochrome P450 2E1|4-nitrophenol 2-hydroxylase|CYPIIE1|cytochrome P450, family 2, subfamily E, polypeptide 1|cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1|cytochrome P450-J|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2E1, check out the CYP2E1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2E1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 2E1 CYP2E1 Antibody (A00672-2)
Hello CJ!
A00672-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhai T,Xu W,Liu Y,Qian K,Xiong Y,Chen Y.Honokiol Alleviates Methionine-Choline Deficient Diet-Induced Hepatic Steatosis and Oxidative Stress in C57BL/6 Mice by Regulating CFLAR-JNK Pathway.Oxid Med Cell Longev.2020 Nov 27;2020:2313641.doi:10.1155/2020/231
Species: Mouse
A00672-2 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cytochrome P450 2E1 CYP2E1 Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-Cytochrome P450 2E1 CYP2E1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question