Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody

HLA-DRB1 antibody

Boster Bio Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (Catalog # M00568). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey.

Product Info Summary

SKU: M00568
Size: 100ug/vial
Reactive Species: Human, Monkey
Host: Mouse
Application: Flow Cytometry, IF, IHC, WB

Product Name

Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody

View all HLA-DRB1 Antibodies

SKU/Catalog Number

M00568

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (Catalog # M00568). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-HLA-DRB (MHC II) HLA-DRB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00568)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM423

Isotype

IgG2b, kappa

Immunogen

Activated human peripheral blood mononuclear cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00568 is reactive to HLA-DRB1 in Human, Monkey

Applications

M00568 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

29.966kDa

Background of HLA-DRB1

This monoclonal antibody reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For HLA-DRB1 (Source: Uniprot.org, NCBI)

Gene Name

HLA-DRB1

Full Name

HLA class II histocompatibility antigen, DRB1 beta chain

Weight

29.966kDa

Alternative Names

Clone P2-beta-3; DR1; DR-1; DR12; DR-12; DR13; DR-13; DR14; DR-14; DR16; DR-16; DR4; DR-4; DR5; DR-5; DR7; DR-7; DR8; DR-8; DR9; DR-9; DRB1; DRw10; DRw11; DRw8; DW2.2/DR2.2; FLJ75017; FLJ76359; HLA class II antigen beta chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA-DR1B; HLA-DRB; HLA-DRB1*; HLA-DRB2; HLA-DR-beta 1; human leucocyte antigen DRB1; leucocyte antigen DR beta 1 chain; leucocyte antigen DRB1; lymphocyte antigen DRB1; major histocompatibility complex, class II, DR beta 1; MHC class II antigen DRB1*1; MHC class II antigen DRB1*10; MHC class II antigen DRB1*11; MH HLA-DRB1 DRB1, HLA-DR1B, HLA-DRB, SS1 major histocompatibility complex, class II, DR beta 1 major histocompatibility complex, class II, DR beta 1 precursor|HLA class II histocompatibility antigen, DR-1 beta chain|MHC class II HLA-DR beta 1 chain|human leucocyte antigen DRB1|lymphocyte antigen DRB1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HLA-DRB1, check out the HLA-DRB1 Infographic

HLA-DRB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HLA-DRB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00568-100ug

100 μg

$468

Free Secondary Antibody

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M00568
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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