Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody

Product Name

Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody

View all ICAM-1/CD54 Antibodies

SKU/Catalog Number

M00171-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody (Catalog# M00171-2). Tested in WB, IHC-P, ELISA, Flow Cytometry, ICC application(s). This antibody reacts with Human, Cow, Rat.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00171-2)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-111

Isotype

Mouse IgG2a

Immunogen

Raji human Burkitt's lymphoma cell line. The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00171-2 is reactive to ICAM1 in Cow, Human, Rat

Applications

M00171-2 is guaranteed for ELISA, Flow Cytometry, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

57.825kDa

Background of ICAM-1/CD54

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 0.5-4 μg/ml.
Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: thymus, RE cells.
Western blotting: Positive control: RAJI cell line K562 leukemia cell line, JY cell line, aa ctivated T-lymphocytes, non-reducing conditions.

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody M00171-2

Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111. Lower bands represent tubulin as a loading control.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For ICAM1 (Source: Uniprot.org, NCBI)

Alternative Names

BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor

For more info on ICAM1, check out the ICAM1 Infographic

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In this infographic, you will see the following information for ICAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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