Anti-Hu CD62P Purified SELP Monoclonal Antibody

Product Name

Anti-Hu CD62P Purified SELP Monoclonal Antibody

View all P-Selectin/CD62P Antibodies

SKU/Catalog Number

M01241-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD62P Purified SELP Monoclonal Antibody (Catalog# M01241-1). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human, Primate.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD62P Purified SELP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01241-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

AK4

Isotype

Mouse IgG1 kappa

Immunogen

Human platelets. The antibody AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01241-1 is reactive to SELP in Human, Primate

Applications

M01241-1 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

90.834kDa

Background of P-Selectin/CD62P

CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1.5 μg/ml.

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of SELP using Anti-Hu CD62P Purified SELP Monoclonal Antibody (M01241-1).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD62P (AK4) purified antibody (concentration in sample 1 μg/ml) GAM APC.

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Figure 2. Flow Cytometry validation of SELP using Anti-Hu CD62P Purified SELP Monoclonal Antibody (M01241-1).

Separation of human thrombocytes (red-filled) from CD62P negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62P (AK4) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Gene/Protein Information For SELP (Source: Uniprot.org, NCBI)

Alternative Names

CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP SELP CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL selectin P P-selectin|CD62 antigen-like family member P|GMP-140|granule membrane protein 140|granule membrane protein 140kDa|granulocyte membrane protein|leukocyte-endothelial cell adhesion molecule 3|platelet activation dependent granule-external membrane protein|platelet alpha-granule membrane protein|selectin P (granule membrane protein 140kDa, antigen CD62)

For more info on SELP, check out the SELP Infographic

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In this infographic, you will see the following information for SELP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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