Click image to see more details
Product Info Summary
| SKU: | FC00171-APC |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD54 Icam1 Monoclonal Antibody APC Conjugated, Flow Validated
View all ICAM-1/CD54 Antibodies
SKU/Catalog Number
FC00171-APC
Size
100 tests, 200 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD54 Icam1 Monoclonal Antibody APC Conjugated, Flow Validated (Catalog# FC00171-APC). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD54 Icam1 Monoclonal Antibody APC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00171-APC)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: LB-2
Isotype
IgG2b,k
Immunogen
MHC Class II antigen negative human Blymphoblastoid cell line
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00171-APC is reactive to ICAM1 in Human
Applications
FC00171-APC is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
57.825kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Daudi gated cells stained with APC conjugated mouse anti-human CD54 (clone LB-2) (red histogram). Daudi gated cells stained with APC conjugated mouse IgG2b isotype control (Blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For ICAM1 (Source: Uniprot.org, NCBI)
Gene Name
ICAM1
Full Name
Intercellular adhesion molecule 1
Weight
57.825kDa
Superfamily
immunoglobulin superfamily
Alternative Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ICAM1, check out the ICAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ICAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD54 Icam1 Monoclonal Antibody APC Conjugated, Flow Validated (FC00171-APC)
Hello CJ!
FC00171-APC has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells
Cheng Y, Zhang Y, Song H, Feng J. Neural Regen Res. 2012 Jun 15;7(17):1312-7. Doi: 10.3969/J.Issn.1673-5374.2012.17.005. Intercellular Adhesion Molecule-1 Expression In The Hippocampal Ca1 Region Of Hyperlipidemic Rats With Chronic Cerebral Ischemia.
Yao Jh, Zhang Xs, Zheng Ss, Li Yh, Wang Lm, Wang Zz, Chu L, Hu Xw, Liu Kx, Tian Xf. World J Gastroenterol. 2009 Jul 14;15(26):3240-5. Prophylaxis With Carnosol Attenuates Liver Injury Induced By Intestinal Ischemia/Reperfusion.
Wang W, Zhao F, Fang Y, Li X, Shen L, Cao T, Zhu H. Crit Care. 2013 Mar 11;17(2):R44. Doi: 10.1186/Cc12558. Glycyrrhizin Protects Against Porcine Endotoxemia Through Modulation Of Systemic Inflammatory Response.
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
11 Customer Q&As for Anti-Human CD54 Icam1 Monoclonal Antibody APC Conjugated, Flow Validated
Question
I see that the anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-06
Question
Will anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC work for Flow Cytometry with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
According to the expression profile of leukemic t-cell, ICAM1 is highly expressed in leukemic t-cell. So, it is likely that anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC will work for Flow Cytometry with leukemic t-cell.
Boster Scientific Support
Answered: 2020-01-06
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
Is this FC00171-APC anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated reactive to the isotypes of ICAM1?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of FC00171-APC anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated is MHC Class II antigen negative human Blymphoblastoid cell line. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
I am looking for to test anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC on human leukemic t-cell for research purposes, then I may be interested in using anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
The products we sell, including anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-20
Question
Will FC00171-APC anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
It shows on the product datasheet, FC00171-APC anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-25
Question
Do you have a BSA free version of anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC available?
N. Li
Verified customer
Asked: 2018-06-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated FC00171-APC. Let me know if you need anything else.
Z. Anderson
Verified customer
Asked: 2015-06-10
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-10
Question
Is a blocking peptide available for product anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated (FC00171-APC)?
K. Parker
Verified customer
Asked: 2015-01-27
Answer
We do provide the blocking peptide for product anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated (FC00171-APC). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-01-27
Question
I have a question about product FC00171-APC, anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Johnson
Verified customer
Asked: 2013-10-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00171-APC anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-10-11
Question
I was wanting to use your anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated for Flow Cytometry for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
A. Miller
Verified customer
Asked: 2013-03-06
Answer
As indicated on the product datasheet, FC00171-APC anti-Human CD54 Monoclonal antibody APC Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-03-06
