Anti-IGF-1R (Ab-1161) Antibody

Product Name

Anti-IGF-1R (Ab-1161) Antibody

View all IGF-I R/IGF1R Antibodies

SKU/Catalog Number

A00070-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-IGF-1R (Ab-1161) Antibody (Catalog # A00070-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IGF-1R (Ab-1161) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00070-1)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Clone Number

1E12E1

Isotype

IgG

Immunogen

Peptide sequence around aa.1159~1163 (D-I-Y-E-T) derived from Human IGF-1R .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00070-1 is reactive to IGF1R in Human, Mouse, Rat

Applications

A00070-1 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

154.793kDa

Background of IGF-I R/IGF1R

This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.

Li S, et al. (1994) J Biol Chem; 269(51).
Hernandez-Sanchez C, et al. (1995) J Biol Chem.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 200 95 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of IGF1R using anti-IGF1R antibody (A00070-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF1R antigen affinity purified polyclonal antibody (Catalog # A00070-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IGF1R.

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Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R(Ab-1161) Antibody #A00070-1.

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Figure 2. IHC analysis of IGF1R using anti-IGF1R antibody (A00070-1).
IGF1R was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IGF1R Antibody (A00070-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For IGF1R (Source: Uniprot.org, NCBI)

Alternative Names

CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2 IGF1R CD221, IGFIR, IGFR, JTK13 insulin like growth factor 1 receptor insulin-like growth factor 1 receptor|IGF-I receptor|soluble IGF1R variant 1|soluble IGF1R variant 2

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